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Literature summary for 2.7.7.4 extracted from

  • Lansdon, E.B.; Fisher, A.J.; Segel, I.H.
    Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains (2004), Biochemistry, 43, 4356-4365.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate competitive with ATP and molybdate Homo sapiens
ATP
-
Homo sapiens
molybdate
-
Homo sapiens
sulfate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
adenylylsulfate pH 8.0, 30°C Homo sapiens
0.0346
-
diphosphate pH 8.0, 30°C Homo sapiens
0.1
-
ATP pH 8.0, 30°C, reaction with molybdate Homo sapiens
0.15
-
ATP pH 8.0, 30°C, reaction with sulfate Homo sapiens
0.16
-
sulfate pH 8.0, 30°C Homo sapiens
1.3
-
molybdate pH 8.0, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43252 recombinant
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00012
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MoO42-
-
Homo sapiens AMP + adenylylmolybdate
-
?
ATP + sulfate
-
Homo sapiens diphosphate + adenylylsulfate
-
?

Subunits

Subunits Comment Organism
More native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate sulfurylase and an adenosine 5'-hosphosulfate kinase domain, catalyzes the overall formation of PAPS from ATP and inorganic sulfate Homo sapiens

Synonyms

Synonyms Comment Organism
adenosine triphosphate sulfurylase native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate sulfurylase and an adenosine 5'-hosphosulfate kinase domain, catalyzes the overall formation of PAPS from ATP and inorganic sulfate Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000018
-
adenosine 5'-phosphosulfate pH 8.0, 30°C Homo sapiens
1
-
ATP pH 8.0, 30°C, reaction with molybdate Homo sapiens
1.1
-
ATP pH 8.0, 30°C, reaction with sulfate Homo sapiens
1.7
-
sulfate pH 8.0, 30°C Homo sapiens
14.3
-
molybdate pH 8.0, 30°C Homo sapiens