Cloned (Comment) | Organism |
---|---|
ATP sulfurylase cDNA from MET3 on chromosome X is amplified and expressed in Escherichia coli XL1-Blue. The synthesis of the enzyme is directed by an expression system that employs the regulatory genes of Vibrio fischeri | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59300 | - |
8 * 59300, SDS-PAGE | Saccharomyces cerevisiae |
470000 | - |
recombinant enzyme, gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | - |
Saccharomyces cerevisiae | diphosphate + adenylylsulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 59300, SDS-PAGE | Saccharomyces cerevisiae |