Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL-21 | Aquifex aeolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ClO3- | - |
Aquifex aeolicus | |
ClO4- | competitive with sulfate and adenylyl sulfate | Aquifex aeolicus | |
FSO3- | - |
Aquifex aeolicus | |
NO3- | - |
Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
adenosine 5'-phosphosulfate | pH 8.0, 37°C | Aquifex aeolicus | |
0.057 | - |
diphosphate | pH 8.0, 30°C | Aquifex aeolicus | |
0.15 | - |
MoO42- | pH 8.0, 30°C | Aquifex aeolicus | |
0.23 | - |
MgATP2- | pH 8.0, 30°C, reaction with MoO42- | Aquifex aeolicus | |
1.1 | - |
MgATP2- | pH 8.0, 30°C, reaction with SO42- | Aquifex aeolicus | |
3.3 | - |
SO42- | pH 8.0, 30°C | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62800 | - |
4 * 62800, calculation from amino acid sequence | Aquifex aeolicus |
260000 | - |
gel filtration | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aquifex aeolicus | the enzyme may also function to produce 3'-phosphoadenosine 5'-phosphosulfate for sulfate ester formation or sulfate assimilation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67174 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MoO42- | - |
Aquifex aeolicus | AMP + adenylylmolybdate | - |
? | |
ATP + sulfate | - |
Aquifex aeolicus | diphosphate + adenylylsulfate | - |
r | |
additional information | the enzyme may also function to produce 3'-phosphoadenosine 5'-phosphosulfate for sulfate ester formation or sulfate assimilation | Aquifex aeolicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62800, calculation from amino acid sequence | Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: more than 1 h | Aquifex aeolicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Aquifex aeolicus |