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Literature summary for 2.7.7.39 extracted from

  • Brault, J.P.; Friesen, J.A.
    Characterization of cytidylyltransferase enzyme activity through high performance liquid chromatography (2016), Anal. Biochem., 510, 26-32.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + sn-glycerol 3-phosphate Enterococcus faecalis
-
diphosphate + CDP-glycerol
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q836D9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + sn-glycerol 3-phosphate
-
Enterococcus faecalis diphosphate + CDP-glycerol
-
?
additional information using an assay method for determination of the catalytic activity measuring the production of radiolabeled CDP-glycerol from 14C-labeled sn-glycerol 3-phosphate, and separation of non-radioactive CDP-glycerol from CTP by C18 reverse phase HPLC with a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4 Enterococcus faecalis ?
-
?

Synonyms

Synonyms Comment Organism
cytidylyltransferase
-
Enterococcus faecalis
GCT
-
Enterococcus faecalis
glycerol 3-phosphate cytidylyltransferase
-
Enterococcus faecalis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Enterococcus faecalis

General Information

General Information Comment Organism
evolution the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids Enterococcus faecalis
metabolism the enzyme catalyzes conversion of phosphocholine and CTP to cytidine diphosphocholine (CDP-choline), a step critical for synthesis of the membrane phospholipid phosphatidylcholine Enterococcus faecalis