BRENDA - Enzyme Database show
show all sequences of 2.7.7.39

Characterization of cytidylyltransferase enzyme activity through high performance liquid chromatography

Brault, J.P.; Friesen, J.A.; Anal. Biochem. 510, 26-32 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Enterococcus faecalis
-
diphosphate + CDP-glycerol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterococcus faecalis
Q836D9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
737438
Enterococcus faecalis
diphosphate + CDP-glycerol
-
-
-
?
additional information
using an assay method for determination of the catalytic activity measuring the production of radiolabeled CDP-glycerol from 14C-labeled sn-glycerol 3-phosphate, and separation of non-radioactive CDP-glycerol from CTP by C18 reverse phase HPLC with a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4
737438
Enterococcus faecalis
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Enterococcus faecalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Enterococcus faecalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Enterococcus faecalis
-
diphosphate + CDP-glycerol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
737438
Enterococcus faecalis
diphosphate + CDP-glycerol
-
-
-
?
additional information
using an assay method for determination of the catalytic activity measuring the production of radiolabeled CDP-glycerol from 14C-labeled sn-glycerol 3-phosphate, and separation of non-radioactive CDP-glycerol from CTP by C18 reverse phase HPLC with a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4
737438
Enterococcus faecalis
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Enterococcus faecalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Enterococcus faecalis
General Information
General Information
Commentary
Organism
evolution
the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids
Enterococcus faecalis
metabolism
the enzyme catalyzes conversion of phosphocholine and CTP to cytidine diphosphocholine (CDP-choline), a step critical for synthesis of the membrane phospholipid phosphatidylcholine
Enterococcus faecalis
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids
Enterococcus faecalis
metabolism
the enzyme catalyzes conversion of phosphocholine and CTP to cytidine diphosphocholine (CDP-choline), a step critical for synthesis of the membrane phospholipid phosphatidylcholine
Enterococcus faecalis
Other publictions for EC 2.7.7.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737438
Brault
Characterization of cytidylylt ...
Enterococcus faecalis
Anal. Biochem.
510
26-32
2016
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672399
Fong
Crystal structure of CTP:glyce ...
Staphylococcus aureus
Biochim. Biophys. Acta
1764
63-69
2006
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645246
Badurina
CTP:glycerol 3-phosphate cytid ...
Saccharomyces cerevisiae, Staphylococcus aureus
Biochim. Biophys. Acta
1646
196-206
2003
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645248
Pattridge
Glycerol-3-phosphate cytidylyl ...
Bacillus subtilis
J. Biol. Chem.
278
51863-51871
2003
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645249
Sanker
Negative cooperativity of subs ...
Bacillus subtilis
J. Biol. Chem.
276
37922-37928
2001
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4
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3
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645250
Yim
Crystallization and preliminar ...
Saccharomyces cerevisiae, Staphylococcus aureus
Acta Crystallogr. Sect. D
57
918-920
2001
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645247
Park
Identification of functional c ...
Bacillus subtilis
J. Biol. Chem.
272
15161-15166
1997
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30
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57
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28
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30
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57
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28
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645242
Park
Expression, purification, and ...
Bacillus subtilis
J. Biol. Chem.
268
16648-16654
1993
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7
2
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1
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645243
Pooley
A conditional-lethal mutant of ...
Bacillus subtilis
J. Gen. Microbiol.
137
921-928
1991
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645241
Cheah
Control of synthesis of wall t ...
Bacillus subtilis, Bacillus subtilis W23
Eur. J. Biochem.
118
497-500
1981
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645244
Hussey
Control of teichoic acid synth ...
Bacillus licheniformis
Eur. J. Biochem.
82
169-174
1978
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645245
Anderson
The control of synthesis of ba ...
Bacillus licheniformis
Biochem. J.
136
871-876
1973
2
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645240
Shaw
Phosphorolysis and enzymic syn ...
Bacillus subtilis, Chlorella vulgaris, Escherichia coli, Lactobacillus plantarum, Propionibacterium freudenreichii subsp. shermanii, Saccharomyces cerevisiae, Staphylococcus aureus
Biochem. J.
82
297-312
1962
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6
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8
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7
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65
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3
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65
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