BRENDA - Enzyme Database show
show all sequences of 2.7.7.39

Glycerol-3-phosphate cytidylyltransferase: Structural changes induced by binding of CDP-glycerol and the role of lysine residues in catalysis

Pattridge, K.A.; Weber, C.H.; Friesen, J.A.; Sanker, S.; Kent, C.; Ludwig, M.L.; J. Biol. Chem. 278, 51863-51871 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Bacillus subtilis
Crystallization (Commentary)
Crystallization
Organism
complexed with CDPglycerol
Bacillus subtilis
Engineering
Amino acid exchange
Commentary
Organism
K44A
less active than wild-type enzyme
Bacillus subtilis
K46A
less active than wild-type enzyme
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Bacillus subtilis
-
diphosphate + CDPglycerol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P27623
-
-
Purification (Commentary)
Commentary
Organism
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
645248
Bacillus subtilis
diphosphate + CDPglycerol
-
-
-
?
CTP + sn-glycerol 3-phosphate
-
645248
Bacillus subtilis
diphosphate + CDPglycerol
-
645248
Bacillus subtilis
?
Subunits
Subunits
Commentary
Organism
dimer
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
complexed with CDPglycerol
Bacillus subtilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K44A
less active than wild-type enzyme
Bacillus subtilis
K46A
less active than wild-type enzyme
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Bacillus subtilis
-
diphosphate + CDPglycerol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
645248
Bacillus subtilis
diphosphate + CDPglycerol
-
-
-
?
CTP + sn-glycerol 3-phosphate
-
645248
Bacillus subtilis
diphosphate + CDPglycerol
-
645248
Bacillus subtilis
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Bacillus subtilis
Other publictions for EC 2.7.7.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737438
Brault
Characterization of cytidylylt ...
Enterococcus faecalis
Anal. Biochem.
510
26-32
2016
-
-
-
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-
1
-
2
-
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2
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1
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1
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1
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-
-
2
-
1
-
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-
1
-
-
-
-
2
2
-
-
-
672399
Fong
Crystal structure of CTP:glyce ...
Staphylococcus aureus
Biochim. Biophys. Acta
1764
63-69
2006
-
-
-
1
-
-
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-
-
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4
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1
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-
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-
645246
Badurina
CTP:glycerol 3-phosphate cytid ...
Saccharomyces cerevisiae, Staphylococcus aureus
Biochim. Biophys. Acta
1646
196-206
2003
-
-
1
-
-
-
2
4
-
-
-
2
-
6
-
-
1
1
-
-
-
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2
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
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-
-
2
-
4
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
645248
Pattridge
Glycerol-3-phosphate cytidylyl ...
Bacillus subtilis
J. Biol. Chem.
278
51863-51871
2003
-
-
1
1
2
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645249
Sanker
Negative cooperativity of subs ...
Bacillus subtilis
J. Biol. Chem.
276
37922-37928
2001
-
-
1
-
4
-
-
-
-
-
-
1
-
3
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645250
Yim
Crystallization and preliminar ...
Saccharomyces cerevisiae, Staphylococcus aureus
Acta Crystallogr. Sect. D
57
918-920
2001
-
-
1
1
-
-
-
-
-
-
-
-
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4
-
-
1
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-
-
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1
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1
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1
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-
645247
Park
Identification of functional c ...
Bacillus subtilis
J. Biol. Chem.
272
15161-15166
1997
-
-
1
-
30
-
-
57
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
28
-
-
-
-
-
-
-
-
-
1
-
-
30
-
-
-
-
57
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
28
-
-
-
-
-
-
-
-
-
-
645242
Park
Expression, purification, and ...
Bacillus subtilis
J. Biol. Chem.
268
16648-16654
1993
-
-
1
-
-
-
7
2
1
4
3
1
-
3
-
-
1
1
-
-
1
2
4
1
2
-
1
-
1
-
3
-
-
-
-
-
-
1
-
-
-
-
-
7
-
2
1
4
3
1
-
-
-
1
-
-
1
2
4
1
2
-
1
-
1
-
3
-
-
-
-
-
-
-
645243
Pooley
A conditional-lethal mutant of ...
Bacillus subtilis
J. Gen. Microbiol.
137
921-928
1991
-
-
-
-
-
-
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-
1
-
1
1
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2
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2
1
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-
1
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-
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-
-
-
-
-
-
-
1
-
1
1
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-
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2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
645241
Cheah
Control of synthesis of wall t ...
Bacillus subtilis, Bacillus subtilis W23
Eur. J. Biochem.
118
497-500
1981
-
-
-
-
-
-
-
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2
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7
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-
1
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4
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2
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1
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4
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-
-
-
-
-
-
-
-
-
645244
Hussey
Control of teichoic acid synth ...
Bacillus licheniformis
Eur. J. Biochem.
82
169-174
1978
-
-
-
-
-
-
-
-
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1
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2
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2
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1
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2
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-
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-
-
-
-
645245
Anderson
The control of synthesis of ba ...
Bacillus licheniformis
Biochem. J.
136
871-876
1973
2
-
-
-
-
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-
1
-
-
1
-
1
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2
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2
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1
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645240
Shaw
Phosphorolysis and enzymic syn ...
Bacillus subtilis, Chlorella vulgaris, Escherichia coli, Lactobacillus plantarum, Propionibacterium freudenreichii subsp. shermanii, Saccharomyces cerevisiae, Staphylococcus aureus
Biochem. J.
82
297-312
1962
-
-
-
-
-
-
-
-
-
6
-
8
-
7
-
-
1
-
-
-
-
-
65
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
8
-
-
-
1
-
-
-
-
65
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-