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show all sequences of 2.7.7.39

CTP:glycerol 3-phosphate cytidylyltransferase (TarD) from Staphylococcus aureus catalyzes the cytidylyl transfer via an ordered bi-bi reaction mechanism with micromolar K(m) values

Badurina, D.S.; Zolli-Juran, M.; Brown, E.D.; Biochim. Biophys. Acta 1646, 196-206 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Staphylococcus aureus
Inhibitors
Inhibitors
Commentary
Organism
Structure
CDPglycerol
mixed inhibition with CTP and glycerol 3-phosphate as substrates
Staphylococcus aureus
diphosphate
mixed inhibition with CTP as substrate
Staphylococcus aureus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
glycerol 3-phosphate
pH 8
Staphylococcus aureus
0.036
-
CTP
pH 8
Staphylococcus aureus
9.7
-
diphosphate
pH 8, reverse reaction
Staphylococcus aureus
60
-
CDPglycerol
pH 8, reverse reaction
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Staphylococcus aureus
-
diphosphate + CDPglycerol
-
-
r
CTP + sn-glycerol 3-phosphate
Saccharomyces cerevisiae
-
diphosphate + CDPglycerol
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Staphylococcus aureus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Staphylococcus aureus
Reaction
Reaction
Commentary
Organism
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
mechanism
Staphylococcus aureus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
645246
Staphylococcus aureus
diphosphate + CDPglycerol
-
-
-
r
CTP + sn-glycerol 3-phosphate
-
645246
Saccharomyces cerevisiae
diphosphate + CDPglycerol
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
CDPglycerol
pH 8, reverse reaction
Staphylococcus aureus
2.6
-
CTP
pH 8, forward reaction
Staphylococcus aureus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Staphylococcus aureus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Staphylococcus aureus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CDPglycerol
mixed inhibition with CTP and glycerol 3-phosphate as substrates
Staphylococcus aureus
diphosphate
mixed inhibition with CTP as substrate
Staphylococcus aureus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
glycerol 3-phosphate
pH 8
Staphylococcus aureus
0.036
-
CTP
pH 8
Staphylococcus aureus
9.7
-
diphosphate
pH 8, reverse reaction
Staphylococcus aureus
60
-
CDPglycerol
pH 8, reverse reaction
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Staphylococcus aureus
-
diphosphate + CDPglycerol
-
-
r
CTP + sn-glycerol 3-phosphate
Saccharomyces cerevisiae
-
diphosphate + CDPglycerol
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
645246
Staphylococcus aureus
diphosphate + CDPglycerol
-
-
-
r
CTP + sn-glycerol 3-phosphate
-
645246
Saccharomyces cerevisiae
diphosphate + CDPglycerol
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
CDPglycerol
pH 8, reverse reaction
Staphylococcus aureus
2.6
-
CTP
pH 8, forward reaction
Staphylococcus aureus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Staphylococcus aureus
Other publictions for EC 2.7.7.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737438
Brault
Characterization of cytidylylt ...
Enterococcus faecalis
Anal. Biochem.
510
26-32
2016
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1
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1
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2
2
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672399
Fong
Crystal structure of CTP:glyce ...
Staphylococcus aureus
Biochim. Biophys. Acta
1764
63-69
2006
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1
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4
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645246
Badurina
CTP:glycerol 3-phosphate cytid ...
Saccharomyces cerevisiae, Staphylococcus aureus
Biochim. Biophys. Acta
1646
196-206
2003
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1
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2
4
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2
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6
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1
1
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1
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4
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2
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2
1
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645248
Pattridge
Glycerol-3-phosphate cytidylyl ...
Bacillus subtilis
J. Biol. Chem.
278
51863-51871
2003
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1
1
2
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1
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1
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1
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1
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645249
Sanker
Negative cooperativity of subs ...
Bacillus subtilis
J. Biol. Chem.
276
37922-37928
2001
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1
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4
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1
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3
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1
1
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1
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645250
Yim
Crystallization and preliminar ...
Saccharomyces cerevisiae, Staphylococcus aureus
Acta Crystallogr. Sect. D
57
918-920
2001
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1
1
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4
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1
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645247
Park
Identification of functional c ...
Bacillus subtilis
J. Biol. Chem.
272
15161-15166
1997
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1
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30
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57
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1
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2
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1
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1
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28
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1
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30
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57
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1
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1
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1
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28
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645242
Park
Expression, purification, and ...
Bacillus subtilis
J. Biol. Chem.
268
16648-16654
1993
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1
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7
2
1
4
3
1
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3
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1
1
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1
2
4
1
2
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1
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1
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3
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1
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7
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2
1
4
3
1
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1
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1
2
4
1
2
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1
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1
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3
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645243
Pooley
A conditional-lethal mutant of ...
Bacillus subtilis
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137
921-928
1991
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1
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1
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645241
Cheah
Control of synthesis of wall t ...
Bacillus subtilis, Bacillus subtilis W23
Eur. J. Biochem.
118
497-500
1981
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2
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7
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1
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4
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1
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4
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645244
Hussey
Control of teichoic acid synth ...
Bacillus licheniformis
Eur. J. Biochem.
82
169-174
1978
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645245
Anderson
The control of synthesis of ba ...
Bacillus licheniformis
Biochem. J.
136
871-876
1973
2
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1
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2
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645240
Shaw
Phosphorolysis and enzymic syn ...
Bacillus subtilis, Chlorella vulgaris, Escherichia coli, Lactobacillus plantarum, Propionibacterium freudenreichii subsp. shermanii, Saccharomyces cerevisiae, Staphylococcus aureus
Biochem. J.
82
297-312
1962
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6
-
8
-
7
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-
1
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65
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3
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6
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8
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1
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65
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3
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