BRENDA - Enzyme Database show
show all sequences of 2.7.7.39

Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis

Park, Y.S.; Sweitzer, T.D.; Dixon, J.E.; Kent, C.; J. Biol. Chem. 268, 16648-16654 (1993)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene expressed in Escherichia coli
Bacillus subtilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Bacillus subtilis
CDPglycerol
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
diphosphate
-
Bacillus subtilis
Hg2+
-
Bacillus subtilis
Sn2+
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.23
-
glycerol 3-phosphate
pH 8, 37°C
Bacillus subtilis
3.85
-
CTP
pH 8, 37°C
Bacillus subtilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Fe2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Mg2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Mn2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14800
-
2 * 14800, gene expression in Escherichia coli, SDS-PAGE
Bacillus subtilis
15271
-
2 * 15271, calculated from the nucleotide sequence
Bacillus subtilis
30900
-
gene expression in E. coli, gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Bacillus subtilis
enzyme of teichoic acid synthesis
diphosphate + CDPglycerol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
BR151, gene expressed in Escherichia coli
-
Purification (Commentary)
Commentary
Organism
gene expression in E. coli
Bacillus subtilis
Reaction
Reaction
Commentary
Organism
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
rapid equilibrium random order mechanism
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85.7
-
-
Bacillus subtilis
Storage Stability
Storage Stability
Organism
-20°C, about 20% loss of activity after 1 month
Bacillus subtilis
-80°C, pure enzyme stable for at least 7 months
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
645242
Bacillus subtilis
diphosphate + CDPglycerol
-
-
-
?
CTP + sn-glycerol 3-phosphate
enzyme of teichoic acid synthesis
645242
Bacillus subtilis
diphosphate + CDPglycerol
-
-
-
?
dCTP + glycerol 3-phosphate
about 95% of the activity with CTP
645242
Bacillus subtilis
diphosphate + dCDPglycerol
-
-
-
?
additional information
no substrate: GTP
645242
Bacillus subtilis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 14800, gene expression in Escherichia coli, SDS-PAGE; 2 * 15271, calculated from the nucleotide sequence
Bacillus subtilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
50
-
-
Bacillus subtilis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
not stable for longer than 30 min
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
9.5
-
Bacillus subtilis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
37°C, 4 h, about 30% loss of activity
Bacillus subtilis
7.5
9.5
37°C, 4 h, stable
Bacillus subtilis
10
-
37°C, 4 h, about 10% loss of activity
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene expressed in Escherichia coli
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Bacillus subtilis
CDPglycerol
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
diphosphate
-
Bacillus subtilis
Hg2+
-
Bacillus subtilis
Sn2+
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.23
-
glycerol 3-phosphate
pH 8, 37°C
Bacillus subtilis
3.85
-
CTP
pH 8, 37°C
Bacillus subtilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Fe2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Mg2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Mn2+
divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14800
-
2 * 14800, gene expression in Escherichia coli, SDS-PAGE
Bacillus subtilis
15271
-
2 * 15271, calculated from the nucleotide sequence
Bacillus subtilis
30900
-
gene expression in E. coli, gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + sn-glycerol 3-phosphate
Bacillus subtilis
enzyme of teichoic acid synthesis
diphosphate + CDPglycerol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
gene expression in E. coli
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85.7
-
-
Bacillus subtilis
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, about 20% loss of activity after 1 month
Bacillus subtilis
-80°C, pure enzyme stable for at least 7 months
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + sn-glycerol 3-phosphate
-
645242
Bacillus subtilis
diphosphate + CDPglycerol
-
-
-
?
CTP + sn-glycerol 3-phosphate
enzyme of teichoic acid synthesis
645242
Bacillus subtilis
diphosphate + CDPglycerol
-
-
-
?
dCTP + glycerol 3-phosphate
about 95% of the activity with CTP
645242
Bacillus subtilis
diphosphate + dCDPglycerol
-
-
-
?
additional information
no substrate: GTP
645242
Bacillus subtilis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 14800, gene expression in Escherichia coli, SDS-PAGE; 2 * 15271, calculated from the nucleotide sequence
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
50
-
-
Bacillus subtilis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
not stable for longer than 30 min
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
9.5
-
Bacillus subtilis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
37°C, 4 h, about 30% loss of activity
Bacillus subtilis
7.5
9.5
37°C, 4 h, stable
Bacillus subtilis
10
-
37°C, 4 h, about 10% loss of activity
Bacillus subtilis
Other publictions for EC 2.7.7.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737438
Brault
Characterization of cytidylylt ...
Enterococcus faecalis
Anal. Biochem.
510
26-32
2016
-
-
-
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1
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2
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1
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1
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1
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-
2
-
1
-
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-
1
-
-
-
-
2
2
-
-
-
672399
Fong
Crystal structure of CTP:glyce ...
Staphylococcus aureus
Biochim. Biophys. Acta
1764
63-69
2006
-
-
-
1
-
-
-
-
-
-
-
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-
4
-
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1
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645246
Badurina
CTP:glycerol 3-phosphate cytid ...
Saccharomyces cerevisiae, Staphylococcus aureus
Biochim. Biophys. Acta
1646
196-206
2003
-
-
1
-
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-
2
4
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2
-
6
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1
1
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2
1
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4
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1
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2
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2
1
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645248
Pattridge
Glycerol-3-phosphate cytidylyl ...
Bacillus subtilis
J. Biol. Chem.
278
51863-51871
2003
-
-
1
1
2
-
-
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-
1
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2
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1
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2
1
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1
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1
2
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1
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1
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-
2
1
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-
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-
-
-
-
-
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-
645249
Sanker
Negative cooperativity of subs ...
Bacillus subtilis
J. Biol. Chem.
276
37922-37928
2001
-
-
1
-
4
-
-
-
-
-
-
1
-
3
-
-
1
1
-
-
-
-
1
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1
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4
-
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1
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1
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-
1
-
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-
-
-
-
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-
-
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-
-
-
-
645250
Yim
Crystallization and preliminar ...
Saccharomyces cerevisiae, Staphylococcus aureus
Acta Crystallogr. Sect. D
57
918-920
2001
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
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-
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-
1
-
1
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1
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645247
Park
Identification of functional c ...
Bacillus subtilis
J. Biol. Chem.
272
15161-15166
1997
-
-
1
-
30
-
-
57
-
-
-
1
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2
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1
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1
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28
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1
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30
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57
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1
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1
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1
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28
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-
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-
-
645242
Park
Expression, purification, and ...
Bacillus subtilis
J. Biol. Chem.
268
16648-16654
1993
-
-
1
-
-
-
7
2
1
4
3
1
-
3
-
-
1
1
-
-
1
2
4
1
2
-
1
-
1
-
3
-
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-
-
1
-
-
-
-
-
7
-
2
1
4
3
1
-
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1
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-
1
2
4
1
2
-
1
-
1
-
3
-
-
-
-
-
-
-
645243
Pooley
A conditional-lethal mutant of ...
Bacillus subtilis
J. Gen. Microbiol.
137
921-928
1991
-
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1
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1
1
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2
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1
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1
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1
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1
1
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2
1
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-
1
-
-
-
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-
-
-
-
-
-
-
645241
Cheah
Control of synthesis of wall t ...
Bacillus subtilis, Bacillus subtilis W23
Eur. J. Biochem.
118
497-500
1981
-
-
-
-
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2
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7
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1
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4
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2
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1
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4
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645244
Hussey
Control of teichoic acid synth ...
Bacillus licheniformis
Eur. J. Biochem.
82
169-174
1978
-
-
-
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1
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2
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1
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2
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645245
Anderson
The control of synthesis of ba ...
Bacillus licheniformis
Biochem. J.
136
871-876
1973
2
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1
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1
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1
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1
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1
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645240
Shaw
Phosphorolysis and enzymic syn ...
Bacillus subtilis, Chlorella vulgaris, Escherichia coli, Lactobacillus plantarum, Propionibacterium freudenreichii subsp. shermanii, Saccharomyces cerevisiae, Staphylococcus aureus
Biochem. J.
82
297-312
1962
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6
-
8
-
7
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1
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65
-
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3
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6
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8
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1
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65
-
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3
-
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