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Literature summary for 2.7.7.39 extracted from

  • Park, Y.S.; Sweitzer, T.D.; Dixon, J.E.; Kent, C.
    Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis (1993), J. Biol. Chem., 268, 16648-16654.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene expressed in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Bacillus subtilis
CDPglycerol
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
diphosphate
-
Bacillus subtilis
Hg2+
-
Bacillus subtilis
Sn2+
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.23
-
glycerol 3-phosphate pH 8, 37┬░C Bacillus subtilis
3.85
-
CTP pH 8, 37┬░C Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis
Fe2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis
Mg2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis
Mn2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14800
-
2 * 14800, gene expression in Escherichia coli, SDS-PAGE Bacillus subtilis
15271
-
2 * 15271, calculated from the nucleotide sequence Bacillus subtilis
30900
-
gene expression in E. coli, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + sn-glycerol 3-phosphate Bacillus subtilis enzyme of teichoic acid synthesis diphosphate + CDPglycerol
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
BR151, gene expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
gene expression in E. coli Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol rapid equilibrium random order mechanism Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
85.7
-
-
Bacillus subtilis

Storage Stability

Storage Stability Organism
-20┬░C, about 20% loss of activity after 1 month Bacillus subtilis
-80┬░C, pure enzyme stable for at least 7 months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + sn-glycerol 3-phosphate
-
Bacillus subtilis diphosphate + CDPglycerol
-
?
CTP + sn-glycerol 3-phosphate enzyme of teichoic acid synthesis Bacillus subtilis diphosphate + CDPglycerol
-
?
dCTP + glycerol 3-phosphate about 95% of the activity with CTP Bacillus subtilis diphosphate + dCDPglycerol
-
?
additional information no substrate: GTP Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 14800, gene expression in Escherichia coli, SDS-PAGE Bacillus subtilis
dimer 2 * 15271, calculated from the nucleotide sequence Bacillus subtilis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Bacillus subtilis
50
-
-
Bacillus subtilis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
not stable for longer than 30 min Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 9.5
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
37┬░C, 4 h, about 30% loss of activity Bacillus subtilis
7.5 9.5 37┬░C, 4 h, stable Bacillus subtilis
10
-
37┬░C, 4 h, about 10% loss of activity Bacillus subtilis