Cloned (Comment) | Organism |
---|---|
gene expressed in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Bacillus subtilis | |
CDPglycerol | - |
Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
diphosphate | - |
Bacillus subtilis | |
Hg2+ | - |
Bacillus subtilis | |
Sn2+ | - |
Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.23 | - |
glycerol 3-phosphate | pH 8, 37°C | Bacillus subtilis | |
3.85 | - |
CTP | pH 8, 37°C | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
Fe2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
Mg2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis | |
Mn2+ | divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14800 | - |
2 * 14800, gene expression in Escherichia coli, SDS-PAGE | Bacillus subtilis |
15271 | - |
2 * 15271, calculated from the nucleotide sequence | Bacillus subtilis |
30900 | - |
gene expression in E. coli, gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + sn-glycerol 3-phosphate | Bacillus subtilis | enzyme of teichoic acid synthesis | diphosphate + CDPglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
BR151, gene expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
gene expression in E. coli | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol | rapid equilibrium random order mechanism | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85.7 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, about 20% loss of activity after 1 month | Bacillus subtilis |
-80°C, pure enzyme stable for at least 7 months | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + sn-glycerol 3-phosphate | - |
Bacillus subtilis | diphosphate + CDPglycerol | - |
? | |
CTP + sn-glycerol 3-phosphate | enzyme of teichoic acid synthesis | Bacillus subtilis | diphosphate + CDPglycerol | - |
? | |
dCTP + glycerol 3-phosphate | about 95% of the activity with CTP | Bacillus subtilis | diphosphate + dCDPglycerol | - |
? | |
additional information | no substrate: GTP | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 14800, gene expression in Escherichia coli, SDS-PAGE | Bacillus subtilis |
dimer | 2 * 15271, calculated from the nucleotide sequence | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
50 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
not stable for longer than 30 min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 9.5 | - |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
37°C, 4 h, about 30% loss of activity | Bacillus subtilis |
7.5 | 9.5 | 37°C, 4 h, stable | Bacillus subtilis |
10 | - |
37°C, 4 h, about 10% loss of activity | Bacillus subtilis |