Any feedback?
Literature summary for 2.7.7.38 extracted from
- A study of Rose Bengal against a 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase as an antibiotic candidate (2020), J. Enzyme Inhib. Med. Chem., 35, 1414-1421 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Burkholderia thailandensis |
| expressed in Escherichia coli BL21(DE3) cells | Chlamydia psittaci |
| expressed in Escherichia coli BL21(DE3) cells | Burkholderia pseudomallei |
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein | Rose Bengal, competitive with 2-keto-3-deoxy-D-manno-octulosonate but non-competitive against CTP | Burkholderia pseudomallei | |
| 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein | Rose Bengal, competitive with 2-keto-3-deoxy-D-manno-octulosonate but non-competitive against CTP | Burkholderia thailandensis | |
| 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein | Rose Bengal, competitive with 2-keto-3-deoxy-D-manno-octulosonate but non-competitive against CTP | Pseudomonas aeruginosa | |
| additional information | not inhibited by 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein | Chlamydia psittaci |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
3-deoxy-D-manno-octulosonate | at pH 7.5 and 25°C | Burkholderia thailandensis |  |
| 0.059 | - |
3-deoxy-D-manno-octulosonate | at pH 7.5 and 25°C | Burkholderia pseudomallei | |
| 0.061 | - |
3-deoxy-D-manno-octulosonate | at pH 7.5 and 25°C | Pseudomonas aeruginosa | |
| 0.0983 | - |
CTP | at pH 7.5 and 25°C | Pseudomonas aeruginosa | |
| 0.1111 | - |
CTP | at pH 7.5 and 25°C | Burkholderia pseudomallei | |
| 0.1239 | - |
CTP | at pH 7.5 and 25°C | Burkholderia thailandensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Burkholderia thailandensis | |
| Mg2+ | 10 mM used in assay conditions | Chlamydia psittaci | |
| Mg2+ | 10 mM used in assay conditions | Burkholderia pseudomallei | |
| Mg2+ | 10 mM used in assay conditions | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3-deoxy-D-manno-octulosonate | Burkholderia thailandensis | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | Chlamydia psittaci | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | Burkholderia pseudomallei | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | Pseudomonas aeruginosa | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | Burkholderia pseudomallei K96243 | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia pseudomallei | Q63WL5 | - |
- |
| Burkholderia pseudomallei K96243 | Q63WL5 | - |
- |
| Burkholderia thailandensis | - |
- |
- |
| Chlamydia psittaci | A0A2D2D9V4 | - |
- |
| Pseudomonas aeruginosa | A0A0C7D0B2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hi-trap Q column chromatography | Burkholderia thailandensis |
| Hi-trap Q column chromatography | Chlamydia psittaci |
| Hi-trap Q column chromatography | Burkholderia pseudomallei |
| Hi-trap Q column chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3-deoxy-D-manno-octulosonate | - |
Burkholderia thailandensis | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | - |
Chlamydia psittaci | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | - |
Burkholderia pseudomallei | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | - |
Pseudomonas aeruginosa | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
| CTP + 3-deoxy-D-manno-octulosonate | - |
Burkholderia pseudomallei K96243 | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29500, calculated from amino acid sequence | Pseudomonas aeruginosa |
| ? | x * 28500, calculated from amino acid sequence | Burkholderia thailandensis |
| ? | x * 30400, calculated from amino acid sequence | Chlamydia psittaci |
| ? | x * 30000, calculated from amino acid sequence | Burkholderia pseudomallei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase | - |
Burkholderia thailandensis |
| 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase | - |
Chlamydia psittaci |
| 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase | - |
Burkholderia pseudomallei |
| 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase | - |
Pseudomonas aeruginosa |
| KdsB | - |
Burkholderia thailandensis |
| KdsB | - |
Chlamydia psittaci |
| KdsB | - |
Burkholderia pseudomallei |
| KdsB | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 76.8 | - |
melting temperature of the native enzyme | Burkholderia pseudomallei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
3-deoxy-D-manno-octulosonate | at pH 7.5 and 25°C | Pseudomonas aeruginosa | |
| 0.088 | - |
3-deoxy-D-manno-octulosonate | at pH 7.5 and 25°C | Burkholderia pseudomallei | |
| 0.116 | - |
3-deoxy-D-manno-octulosonate | at pH 7.5 and 25°C | Burkholderia thailandensis | |
| 0.132 | - |
CTP | at pH 7.5 and 25°C | Pseudomonas aeruginosa | |
| 0.142 | - |
CTP | at pH 7.5 and 25°C | Burkholderia thailandensis | |
| 0.167 | - |
CTP | at pH 7.5 and 25°C | Burkholderia pseudomallei | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00531 | - |
at pH 7.5 and 25°C | Burkholderia pseudomallei | 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein | |
| 0.00724 | - |
at pH 7.5 and 25°C | Burkholderia thailandensis | 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein | |
| 0.01466 | - |
at pH 7.5 and 25°C | Pseudomonas aeruginosa | 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
