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Literature summary for 2.7.7.38 extracted from

  • Schmidt, H.; Mesters, J.; Wu, J.; Woodard, R.; Hilgenfeld, R.; Mamat, U.
    Evidence for a two-metal-ion mechanism in the cytidyltransferase KdsB, an enzyme involved in lipopolysaccharide biosynthesis (2011), PLoS ONE, 6, e23231.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.38

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.1 A resolution. Two Mg2+ ions are part of the active site of the enzyme Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 CTP pH 9.0, 50°C Aquifex aeolicus
0.452
-
 3-deoxy-D-manno-octulosonate pH 9.0, 50°C Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ two Mg2+ ions are part of the active site of the enzyme Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE Aquifex aeolicus
53000
-
 gel filtration Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O66914
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate
-
 Aquifex aeolicus diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
additional information no substrates: ATP, GTP Aquifex aeolicus ?
-
 ?
UTP + 3-deoxy-D-manno-octulosonate about 45% of the activity with CTP Aquifex aeolicus diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE Aquifex aeolicus

Synonyms

Synonyms Comment Organism
CKS
-
 Aquifex aeolicus
CMP-KDO synthetase
-
 Aquifex aeolicus
KdsB
-
 Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Aquifex aeolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 95
-
 Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.5
-
 3-deoxy-D-manno-octulosonate pH 9.0, 50°C Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aquifex aeolicus

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5
-
 Aquifex aeolicus