Application | Comment | Organism |
---|---|---|
analysis | adaptation of a simple colorimetric assay for diphosphate to the enzyme 3-deoxy-D-manno-octulosonate cytidylyltransferase. This assay can be combined with the malachite green assay for phosphate to form an assay system capable of determining phosphate and diphosphate in the same solution. The assay system has the potential for simultaneous screening of the 3-deoxy-D-manno-octulosonate biosynthesis pathway | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
3-deoxy-D-manno-octulosonate | in the presence of 10 mM Mg2+, at pH 9.3 and 37°C | Escherichia coli | |
0.33 | - |
CTP | in the presence of 10 mM Mg2+, at pH 9.3 and 37°C | Escherichia coli | |
0.52 | - |
3-deoxy-D-manno-octulosonate | in the presence of 5 mM Mg2+, at pH 10.0 and 37°C | Escherichia coli | |
0.59 | - |
CTP | in the presence of 5 mM Mg2+, at pH 10.0 and 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimum activity at 10 mM Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli | CMP-3-deoxy-D-manno-octulosonate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-manno-octulosonate cytidylyltransferase | - |
Escherichia coli |
CMP-KDO synthetase | - |
Escherichia coli |
KdsB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
3-deoxy-D-manno-octulosonate | in the presence of 10 mM Mg2+, at pH 9.3 and 37°C | Escherichia coli | |
21.9 | - |
3-deoxy-D-manno-octulosonate | in the presence of 5 mM Mg2+, at pH 10.0 and 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Escherichia coli |