Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.38 extracted from

  • Yi, L.; Velasquez, M.S.; Holler, T.P.; Woodard, R.W.
    A simple assay for 3-deoxy-D-manno-octulosonate cytidylyltransferase and its use as a pathway screen (2011), Anal. Biochem., 416, 152-158.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis adaptation of a simple colorimetric assay for diphosphate to the enzyme 3-deoxy-D-manno-octulosonate cytidylyltransferase. This assay can be combined with the malachite green assay for phosphate to form an assay system capable of determining phosphate and diphosphate in the same solution. The assay system has the potential for simultaneous screening of the 3-deoxy-D-manno-octulosonate biosynthesis pathway Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
3-deoxy-D-manno-octulosonate in the presence of 10 mM Mg2+, at pH 9.3 and 37°C Escherichia coli
0.33
-
CTP in the presence of 10 mM Mg2+, at pH 9.3 and 37°C Escherichia coli
0.52
-
3-deoxy-D-manno-octulosonate in the presence of 5 mM Mg2+, at pH 10.0 and 37°C Escherichia coli
0.59
-
CTP in the presence of 5 mM Mg2+, at pH 10.0 and 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum activity at 10 mM Mg2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli CMP-3-deoxy-D-manno-octulosonate + diphosphate
-
?

Synonyms

Synonyms Comment Organism
3-deoxy-D-manno-octulosonate cytidylyltransferase
-
Escherichia coli
CMP-KDO synthetase
-
Escherichia coli
KdsB
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.5
-
3-deoxy-D-manno-octulosonate in the presence of 10 mM Mg2+, at pH 9.3 and 37°C Escherichia coli
21.9
-
3-deoxy-D-manno-octulosonate in the presence of 5 mM Mg2+, at pH 10.0 and 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
Escherichia coli