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Literature summary for 2.7.7.38 extracted from

  • Ray, P.H.; Benedict, C.D.; Grasmuk, H.
    Purification and characterization of cytidine 5-triphosphate:cytidine 5-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase (1981), J. Bacteriol., 145, 1273-1280.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.38

Inhibitors

Inhibitors Comment Organism Structure
diphosphate weak inhibition Escherichia coli
Hg2+ strong inhibition Escherichia coli
additional information no inhibition by CDP, CMP, 3-deoxy-manno-octulosonate 8-phosphate, N-acetylneuraminate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 CTP
-
 Escherichia coli
0.29
-
 3-deoxy-D-manno-octulosonate pH 9.5, 30°C Escherichia coli
0.34
-
 dCTP pH 8.0, 37°C Escherichia coli
0.88
-
 UTP pH 8.0, 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activation, 20% as effective as Mg2+ Escherichia coli
Ca2+ activation, 14% as effective as Mg2+ Escherichia coli
Cd2+ activation, 42% as effective as Mg2+ Escherichia coli
Co2+ activation, 11% as effective as Mg2+ Escherichia coli
Mg2+ requires 10 mM for maximal activity, other divalent cations including Mn2+ and Co2+ are much less effective Escherichia coli
Mn2+ activation, 38% as effective as Mg2+ Escherichia coli
additional information monovalent cations Na+, Li+ andK+ are ineffective in stimulating the enzyme, as are divalent cations Hg2+, Ni2+, and Fe2+ Escherichia coli
Zn2+ activation, 32% as effective as Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000 45000 sucrose density gradient centrifugation Escherichia coli
35000 40000 SDS-PAGE Escherichia coli
36000
-
 1 * 36000, SDS-PAGE Escherichia coli
40000 46000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli B / ATCC 11303
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.9
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-90°C, partially purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, stable for up to 3 months Escherichia coli
4°C, partially purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, stable for up to 3 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli B / ATCC 11303 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 and 9.5 Escherichia coli diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
 r
dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 and 9.5 Escherichia coli B / ATCC 11303 diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
 r
additional information no other sugar can replace 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate and N-acetylneuraminic acid will not serve as substrates, neither dUTP nor any other nucleotide triphosphates, diphosphates or monophosphates like ATP, dATP, GTP, dGTP, ITP, dITP, TTP, CDP, UDP or CMP can substitute for CTP Escherichia coli ?
-
 ?
additional information no other sugar can replace 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate and N-acetylneuraminic acid will not serve as substrates, neither dUTP nor any other nucleotide triphosphates, diphosphates or monophosphates like ATP, dATP, GTP, dGTP, ITP, dITP, TTP, CDP, UDP or CMP can substitute for CTP Escherichia coli B / ATCC 11303 ?
-
 ?
UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, to a lesser extent at pH 8.0 Escherichia coli diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 r
UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, to a lesser extent at pH 8.0 Escherichia coli B / ATCC 11303 diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
 0.2 M glycine NaOH buffer Escherichia coli
9.6
-
 0.2 M Tris-acetate buffer Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.2 about half-maximal activity at pH 7.5 and about 80% of maximal activity at pH 10.2 in Tris-acetate buffer Escherichia coli
7.5 10.5 about half-maximal activity at pH 7.5 and about 60% of maximal activity at pH 10.5 in glycine-NaOH buffer Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4 8 stable during purification in Tris-acetate or Tris-chloride buffer, more stable in phosphate buffer than in Tris-acetate or Tris-chloride buffer Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing 4.4 4.15