Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.34 extracted from

  • Danishefsky, I.; Heritier-Watkins, O.
    Nucleoside diphosphate glucose pyrophosphorylases in mast cell tumors (1967), Biochim. Biophys. Acta, 139, 349-357.
    View publication on PubMed
Search for more literature for 2.7.7.34

Inhibitors

Inhibitors Comment Organism Structure
D-mannose 1-phosphate 0.07 mM: 18% inhibition in presence of 3.5 mM GDPglucose, 40% inhibition in presence of 0.7 mM GDPglucose, no inhibition in presence of more than 7 mM GDPglucose Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 GDPglucose pH 7.4, 30°C Mus musculus
0.8
-
 diphosphate pH 7.4, 30°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement, highest activity at 8-10 mM Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus involved in biosynthesis of heparin in mast cell tissue ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 mast cell tumor, grown in LAF mice
-

Purification (Commentary)

Purification (Comment) Organism
mast cell tumor, grown in LAF mice, partial Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
additional information no activity in fibrosarcomase and umbilical cord Mus musculus
-
myocytoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.046
-
-
 Mus musculus

Storage Stability

Storage Stability Organism
-20°C, dried acetone powder, stable for at least two weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-glucose 1-phosphate
-
 Mus musculus diphosphate + GDP-glucose
-
 r
GTP + D-mannose 1-phosphate less effective than alpha-D-glucose 1-phosphate Mus musculus diphosphate + GDP-mannose
-
 r
additional information ATP, CTP, TTP, UTP are ineffective Mus musculus ?
-
 ?
additional information involved in biosynthesis of heparin in mast cell tissue Mus musculus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.7
-
 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.7 pH 6: 65% of maximal activity, pH 8.7: 60% of maximal activity at pH 8.7 Mus musculus