Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.33 extracted from

  • Qi, X.Q.; Sun, Q.L.; Bai, L.P.; Shan, J.J.; Zhang, Y.; Zhang, R.; Li, Y.
    Identification of alpha-D-glucose-1-phosphate cytidylyltransferase involved in Ebosin biosynthesis of Streptomyces sp. 139 (2009), Appl. Microbiol. Biotechnol., 83, 361-368.
    View publication on PubMed

Application

Application Comment Organism
medicine ebosin is an exopolysaccharide with antagonist activity for interleukin-1 receptor in vitro and remarkable anti-rheumatic arthritis activity in vivo Streptomyces sp. 139

Cloned(Commentary)

Cloned (Comment) Organism
the ste17 gene coding region is cloned into the plasmid pET30a for expression in Escherichia coli BL21 cells Streptomyces sp. 139

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.142
-
CTP
-
Streptomyces sp. 139
0.429
-
alpha-D-glucose 1-phosphate
-
Streptomyces sp. 139

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30900
-
including 2.5 kDa of His-tag originated from pET30a, determined by SDS-PAGE Streptomyces sp. 139

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + alpha-D-glucose 1-phosphate Streptomyces sp. 139
-
diphosphate + CDP-glucose
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. 139 Q8KN04
-
-

Purification (Commentary)

Purification (Comment) Organism
on a Ni-NTA His-Bind resin column Streptomyces sp. 139

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + alpha-D-glucose 1-phosphate
-
Streptomyces sp. 139 diphosphate + CDP-glucose
-
?

Synonyms

Synonyms Comment Organism
alpha-D-glucose-1-phosphate cytidylyltransferase
-
Streptomyces sp. 139
CDP-D-glucose synthase
-
Streptomyces sp. 139

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Streptomyces sp. 139

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Streptomyces sp. 139