Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.33 extracted from

  • Koropatkin, N.M.; Cleland, W.W.; Holden, H.M.
    Kinetic and structural analysis of alpha-D-glucose-1-phosphate cytidylyltransferase from Salmonella typhi (2005), J. Biol. Chem., 280, 10774-10780.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method.The structure of the enzyme with bound MgCTP reveals that the binding pocket for the nucleotide is contained within one subunit rather than shared between two. Key side chains involved in nucleotide binding include Thr14, Arg15, Lys25, and Arg111 Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
CDP-glucose
-
Salmonella enterica subsp. enterica serovar Typhi
0.052
-
alpha-D-glucose 1-phosphate
-
Salmonella enterica subsp. enterica serovar Typhi
0.067
-
diphosphate
-
Salmonella enterica subsp. enterica serovar Typhi
0.148
-
CTP
-
Salmonella enterica subsp. enterica serovar Typhi
2.535
-
UDP-glucose
-
Salmonella enterica subsp. enterica serovar Typhi

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + alpha-D-glucose 1-phosphate the structure of the enzyme with bound MgCTP reveals that the binding pocket for the nucleotide is contained within one subunit rather than shared between two. Key side chains involved in nucleotide binding include Thr14, Arg15, Lys25, and Arg111 Salmonella enterica subsp. enterica serovar Typhi diphosphate + CDP-glucose
-
r
UTP + alpha-D-glucose 1-phosphate
-
Salmonella enterica subsp. enterica serovar Typhi diphosphate + UDP-glucose
-
?

Synonyms

Synonyms Comment Organism
alpha-D-glucose-1-phosphate cytidylyltransferase
-
Salmonella enterica subsp. enterica serovar Typhi