Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.31 extracted from

  • Zhou, F.; Cui, X.; Shang, A.; Lian, J.; Yang, L.; Jin, Y.; Li, B.
    Fluorometric determination of the activity and inhibition of terminal deoxynucleotidyl transferase via in-situ formation of copper nanoclusters using enzymatically generated DNA as template (2017), Microchim. Acta, 184, 773-779 .
    View publication on PubMed
Search for more literature for 2.7.7.31

Inhibitors

Inhibitors Comment Organism Structure
aurintricarboxylic acid
-
 Homo sapiens
diphosphate
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 40 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn Homo sapiens the enzyme catalyzes the elongation of a DNA with the length of three or more nucleotides by the isothermal and repetitive addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules diphosphate + single stranded DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
acute lymphoblastic leukemia cell
-
 Homo sapiens
-
lymphocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn the enzyme catalyzes the elongation of a DNA with the length of three or more nucleotides by the isothermal and repetitive addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules Homo sapiens diphosphate + single stranded DNAn+1
-
 ?
dATP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
dTTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
TdT
-
 Homo sapiens
terminal deoxynucleotidyl transferase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 the enzyme is inactivated after heating to 75°C for 10 min Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00053
-
 at pH 7.2 and 37°C Homo sapiens aurintricarboxylic acid
1.27
-
 at pH 7.2 and 37°C Homo sapiens diphosphate