Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.30 extracted from

  • Quirk, S.; Seley, K.L.
    Identification of catalytic amino acids in the human GTP fucose pyrophosphorylase active site (2005), Biochemistry, 44, 13172-13178.
    View publication on PubMed
Search for more literature for 2.7.7.30

Protein Variants

Protein Variants Comment Organism
D184A the ratio of turnover number to Km-value (for GTP) is 1.02fold higher than the wild-type ratio Homo sapiens
D299A the ratio of turnover number to Km-value (for GTP) is 1.04fold higher than the wild-type ratio Homo sapiens
D427A the ratio of turnover number to Km-value (for GTP) is 1.06fold lower than the wild-type ratio Homo sapiens
D77A the ratio of turnover number to Km-value (for GTP) is 16600fold lower than the wild-type ratio Homo sapiens
E289A the ratio of turnover number to Km-value (for GTP) is 1.1fold lower than the wild-type ratio Homo sapiens
E297A the ratio of turnover number to Km-value (for GTP) is 19.5fold lower than the wild-type ratio Homo sapiens
E297A/E432A the ratio of turnover number to Km-value (for GTP) is 24900fold lower than the wild-type ratio Homo sapiens
E432A the ratio of turnover number to Km-value (for GTP) is 113fold lower than the wild-type ratio Homo sapiens
E566A the ratio of turnover number to Km-value (for GTP) is 1.06fold higher than the wild-type ratio Homo sapiens
E574A the ratio of turnover number to Km-value (for GTP) is 1.05fold lower than the wild-type ratio Homo sapiens
K478A the ratio of turnover number to Km-value (for GTP) is 1.13fold lower than the wild-type ratio Homo sapiens
K479A the ratio of turnover number to Km-value (for GTP) is 1.15fold lower than the wild-type ratio Homo sapiens
K482A the ratio of turnover number to Km-value (for GTP) is 166fold lower than the wild-type ratio Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0491
-
 GTP pH 7.0, 37°C, mutant enzyme D427A Homo sapiens
0.0496
-
 GTP pH 7.0, 37°C, mutant enzyme E566A Homo sapiens
0.0501
-
 GTP pH 7.0, 37°C, mutant enzyme D299A Homo sapiens
0.0502
-
 GTP pH 7.0, 37°C, wild-type enzyme Homo sapiens
0.0506
-
 GTP pH 7.0, 37°C, mutant enzyme E574A Homo sapiens
0.0511
-
 GTP pH 7.0, 37°C, mutant enzyme K479A Homo sapiens
0.0512
-
 GTP pH 7.0, 37°C, mutant enzyme D184A Homo sapiens
0.0522
-
 GTP pH 7.0, 37°C, mutant enzyme K478A Homo sapiens
0.0537
-
 GTP pH 7.0, 37°C, mutant enzyme E289A Homo sapiens
0.0805
-
 GTP pH 7.0, 37°C, mutant enzyme E297A Homo sapiens
0.127
-
 GTP pH 7.0, 37°C, mutant enzyme E432A Homo sapiens
0.289
-
 GTP pH 7.0, 37°C, mutant enzyme D77A Homo sapiens
0.422
-
 GTP pH 7.0, 37°C, mutant enzyme E297A/E432A Homo sapiens
0.633
-
 GTP pH 7.0, 37°C, mutant enzyme K482A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + L-fucose 1-phosphate
-
 Homo sapiens diphosphate + GDP-L-fucose
-
 ?

Synonyms

Synonyms Comment Organism
GFPP
-
 Homo sapiens
GTP fucose pyrophosphorylase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 GTP pH 7.0, 37°C, mutant enzyme D77A Homo sapiens
0.001
-
 GTP pH 7.0, 37°C, mutant enzyme E297A/E432A Homo sapiens
0.05
-
 GTP pH 7.0, 37°C, mutant enzyme E432A Homo sapiens
0.19
-
 GTP pH 7.0, 37°C, mutant enzyme K482A Homo sapiens
0.2
-
 GTP pH 7.0, 37°C, mutant enzyme E297A Homo sapiens
2.2
-
 GTP pH 7.0, 37°C, mutant enzyme K479A Homo sapiens
2.3
-
 GTP pH 7.0, 37°C, mutant enzyme K478A Homo sapiens
2.4
-
 GTP pH 7.0, 37°C, mutant enzyme E289A Homo sapiens
2.4
-
 GTP pH 7.0, 37°C, mutant enzyme E574A Homo sapiens
2.5
-
 GTP pH 7.0, 37°C, wild-type enzyme Homo sapiens
2.6
-
 GTP pH 7.0, 37°C, mutant enzyme D184A Homo sapiens
2.6
-
 GTP pH 7.0, 37°C, mutant enzyme D299A Homo sapiens
2.6
-
 GTP pH 7.0, 37°C, mutant enzyme D427A Homo sapiens
2.6
-
 GTP pH 7.0, 37°C, mutant enzyme E566A Homo sapiens