KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
GDP-L-fucose | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Sus scrofa | |
Mg2+ | divalent cation required, Mg2+ most effective | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, ammonium sulfate, calcium phosphate gel, Sephadex G-100, DEAE-cellulose, alumina C-gamma | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0036 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-16°C, 10 mM dithioerythritol, 24 hours, 30-35% loss of activity | Sus scrofa |
-16°C, 24 hours, 80% loss of activity | Sus scrofa |
0°C, 24 hours, 85% loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + GDP-L-fucose | - |
Sus scrofa | GTP + L-fucose 1-phosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | 17.5% of activity with GDP-L-fucose | Sus scrofa | diphosphate + GDP-alpha-D-mannopyranose | - |
? | |
GTP + L-fucose 1-phosphate | specific for GTP | Sus scrofa | diphosphate + GDP-L-fucose | - |
r | |
UTP + L-fucose 1-phosphate | 18.7% of activity with GTP | Sus scrofa | diphosphate + UDP-L-fucose | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | approx. 85% of maximal activity at pH 6.0, approx. 70% of maximal activity at pH 9.5 | Sus scrofa |