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Literature summary for 2.7.7.3 extracted from

  • Cheng, C.S.; Jia, K.F.; Chen, T.; Chang, S.Y.; Lin, M.S.; Yin, H.S.
    Experimentally validated novel inhibitors of Helicobacter pylori phosphopantetheine adenylyltransferase discovered by virtual high-throughput screening (2013), PLoS ONE, 8, e74271.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is an attractive target for antibacterial drug development against Helicobacter pylori Helicobacter pylori
medicine the enzyme is a therapeutical target in treatment of Helicobacter induced gastritis Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli strain BL21(DE3) Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
AC1LDVWJ
-
Helicobacter pylori
D-amethopterin a mixed-type inhibitor of HpPPAT activity, that simultaneously occupies the HpPPAT 4'-phosphopantetheine- and ATP-binding sites, the compound can serve as a lead compound in drug development, steady-state kinetic inhibition assay. Model of HpPPAT complexed with D-amethopterin, overview Helicobacter pylori
additional information inhibitor virtual high-throughput screening using the HpPPAT crystal structure, docking study, overview Helicobacter pylori
Omeprazole
-
Helicobacter pylori
T5568746
-
Helicobacter pylori
zanamivir
-
Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pantetheine 4'-phosphate Helicobacter pylori
-
diphosphate + 3'-dephospho-CoA
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O26010
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pantetheine 4'-phosphate
-
Helicobacter pylori diphosphate + 3'-dephospho-CoA
-
?

Synonyms

Synonyms Comment Organism
phosphopantetheine adenylyltransferase
-
Helicobacter pylori
PPAT
-
Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Helicobacter pylori

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2674
-
D-amethopterin pH 7.9, 25°C, recombinant enzyme Helicobacter pylori

General Information

General Information Comment Organism
malfunction enzyme inactivation effectively prevents bacterial viability Helicobacter pylori
metabolism the enzyme catalyzes the penultimate step in coenzyme A biosynthesis Helicobacter pylori