Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | A0.5 value for wild-type 0.28 mm, for large subunit E370G/small subunit wild-type 0.83 mM | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
E370G | mutation in the large subunit alters the heterotetrameric stability along with the binding properties of substrate and effectors of the enzyme. The affinity of the large subunit E370G/small subunit wild-type AGPase for glucose-1-phosphate is 3fold less than for wild type AGPase. The mutant enzyme complex requires 3fold more 3-phosphogyceric acid to be activated and lis less heat stable | Solanum tuberosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Solanum tuberosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
alpha-D-glucose 1-phosphate | wild-type, pH 7.4, 37°C | Solanum tuberosum | |
0.05 | - |
ATP | wild-type, pH 7.4, 37°C | Solanum tuberosum | |
0.09 | - |
ATP | large subunit E370G/small subunit wild-type, pH 7.4, 37°C | Solanum tuberosum | |
0.13 | - |
alpha-D-glucose 1-phosphate | large subunit E370G/small subunit wild-type, pH 7.4, 37°C | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | Q00081 | large subbunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.15 | - |
large subunit E370G/small subunit wild-type, presence of 1 mM 3-phosphoglycerate, pH 7.4, 37°C | Solanum tuberosum | phosphate | |
0.38 | - |
large subunit E370G/small subunit wild-type, presence of 2.5 mM 3-phosphoglycerate, pH 7.4, 37°C | Solanum tuberosum | phosphate | |
0.44 | - |
wild-type, presence of 1 mM 3-phosphoglycerate, pH 7.4, 37°C | Solanum tuberosum | phosphate | |
0.67 | - |
wild-type, presence of 2.5 mM 3-phosphoglycerate, pH 7.4, 37°C | Solanum tuberosum | phosphate |