Any feedback?
Literature summary for 2.7.7.27 extracted from
- Identification of the ADP-glucose pyrophosphorylase isoforms essential for starch synthesis in the leaf and seed endosperm of rice (Oryza sativa L.) (2007), Plant Mol. Biol., 65, 531-546.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AGPL1, DNA and amino acid sequence determination and analysis, expression analysis in different tissues, transient expression of rice isozymes in Zea mays protoplasts | Oryza sativa |
| gene AGPL2, DNA and amino acid sequence determination and analysis, expression analysis in different tissues, transient expression of rice isozymes in Zea mays protoplasts | Oryza sativa |
| gene AGPL3, DNA and amino acid sequence determination and analysis, expression analysis in different tissues, transient expression of rice isozymes in Zea mays protoplasts | Oryza sativa |
| gene AGPL4, DNA and amino acid sequence determination and analysis, expression analysis in different tissues, transient expression of rice isozymes in Zea mays protoplasts | Oryza sativa |
| gene AGPS1, DNA and amino acid sequence determination and analysis, expression analysis in different tissues, transient expression of rice isozymes in Zea mays protoplasts | Oryza sativa |
| gene AGPS2 a and b, DNA and amino acid sequence determination and analysis, transient expression of rice isozymes in Zea mays protoplasts | Oryza sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | lesions in the cytosolic enzyme isoforms small subunit OsAGPS2b, or large subunit OsAGPL2, cause a shrunken endosperm due to a remarkable reduction in starch synthesis. During vegetative growth, the osagps2 mutant is indstinguishable from wild-type | Oryza sativa |
| additional information | isolation of osagpl2 mutants, subcellular localization, the mutant plants show reduced starch content, phenotypes, overview | Oryza sativa |
| additional information | isolation of osagps2 mutants, subcellular localization, expression analysis in different tissues, the mutant plants show reduced starch content, phenotypes, overview | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | inhibits at higher concentrations; inhibits at higher concentrations; inhibits at higher concentrations; inhibits at higher concentrations; inhibits at higher concentrations; inhibits at higher concentrations | Oryza sativa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | AGPS1 | Oryza sativa | 9507 | - |
| chloroplast | AGPS2 b | Oryza sativa | 9507 | - |
| chloroplast | the N-termini of isozymes AGPL1, AGPL3, and AGPL4 contain a putative transit peptide, the proteins are targeted to the plastid stroma | Oryza sativa | 9507 | - |
| cytosol | seed-specific small subunit OsAGPS2b, and large subunit OsAGPL2 | Oryza sativa | 5829 | - |
| cytosol | AGPS2 a | Oryza sativa | 5829 | - |
| cytosol | isozyme AGPL2 lacks an apparent transit peptide | Oryza sativa | 5829 | - |
| additional information | subcellular localization of AGP isozymes, overview | Oryza sativa | - |
- |
| plastid | large subunit isoforms OsAGPL1, OsAGPL3, OsAGPL4 and both small subunit isoforms OsAGPS1, OsAGPS2 | Oryza sativa | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Oryza sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | Oryza sativa | first committed step of starch biosynthesis in higher plants, identification of identify AGP isoforms essential for this biosynthetic process in sink and source tissues of rice plants | ADP-D-glucose + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
gene family consists of two genes OsAGPS1 and OsAGPS2 encoding enzyme small subunits and four genes OsAGPL1, OsAGPL2, OsAGPL3, OsAGPL4 encoding enzyme large subunits | - |
| Oryza sativa | - |
gene AGPL4, the AGP gene family consists of two genes, OsAGPS1 and OsAGPS2, encoding small subunits and four genes, OsAGPL1, OsAGPL2, OsAGPL3 and OsAGPL4, encoding large subunits of this enzyme heterotetrameric complex | - |
| Oryza sativa | P15280 | gene AGPS2 a and b; the AGP gene family consists of two genes, OsAGPS1 and OsAGPS2, encoding small subunits and four genes, OsAGPL1, OsAGPL2, OsAGPL3 and OsAGPL4, encoding large subunits of this enzyme heterotetrameric complex | - |
| Oryza sativa | Q688T8 | gene AGPL1; gene AGPL1, the AGP gene family consists of two genes, OsAGPS1 and OsAGPS2, encoding small subunits and four genes, OsAGPL1, OsAGPL2, OsAGPL3 and OsAGPL4, encoding large subunits of this enzyme heterotetrameric complex | - |
| Oryza sativa | Q69T99 | gene AGPS1; gene AGPS1, the AGP gene family consists of two genes, OsAGPS1 and OsAGPS2, encoding small subunits and four genes, OsAGPL1, OsAGPL2, OsAGPL3 and OsAGPL4, encoding large subunits of this enzyme heterotetrameric complex | - |
| Oryza sativa | Q6AVT2 | gene AGPL3; gene AGPL3, the AGP gene family consists of two genes, OsAGPS1 and OsAGPS2, encoding small subunits and four genes, OsAGPL1, OsAGPL2, OsAGPL3 and OsAGPL4, encoding large subunits of this enzyme heterotetrameric complex | - |
| Oryza sativa | Q7G065 | gene AGPL2; gene AGPL2, the AGP gene family consists of two genes, OsAGPS1 and OsAGPS2, encoding small subunits and four genes, OsAGPL1, OsAGPL2, OsAGPL3 and OsAGPL4, encoding large subunits of this enzyme heterotetrameric complex | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Oryza sativa | - |
| endosperm | early stage | Oryza sativa | - |
| endosperm | OsAGPS2b and OsAGPL2 are the major isoforms | Oryza sativa | - |
| endosperm | early stages | Oryza sativa | - |
| leaf | - |
Oryza sativa | - |
| leaf | OsAGPS2a is the main small subunit isoform | Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Oryza sativa | ADP-D-glucose + diphosphate | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | first committed step of starch biosynthesis in higher plants, identification of identify AGP isoforms essential for this biosynthetic process in sink and source tissues of rice plants | Oryza sativa | ADP-D-glucose + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | heterotetramer | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP-glucose pyrophosphorylase | - |
Oryza sativa |
| AGP | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Oryza sativa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
