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Literature summary for 2.7.7.27 extracted from

  • Kim, D.; Hwang, S.K.; Okita, T.W.
    Subunit interactions specify the allosteric regulatory properties of the potato tuber ADP-glucose pyrophosphorylase (2007), Biochem. Biophys. Res. Commun., 362, 301-306.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate 50% activation of wild-type at 0.04 mM, of mutation P52L, large subunit, at 0.27 mM, of mutation P52L, large subunit, plus mutation L46F, small subunit at 0.1 mM, of mutation P52L, large subunit, plus mutation P112L, small subunit at 0.14 mM, of mutation P52L, large subunit, plus mutation P308L, small subunit at 0.006 mM, of mutation P52L, large subunit, plus mutation R350K, small subunit at 0.01 mM Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
L46F mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate Solanum tuberosum
P112L mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate Solanum tuberosum
P308L mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate Solanum tuberosum
P52L mutation in large subunit, mutant is 5fold less sensitive to activation by 3-phosphoglycerate Solanum tuberosum
R350K mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
phosphate at 0.5 mM 3-phosphoglycerate, 50% inhibition of wild-type at 0.54 mM, of mutation P52L, large subunit, at 0.52 mM, of mutation P52L, large subunit, plus mutation L46F, small subunit at 0.61 mM, of mutation P52L, large subunit, plus mutation P112L, small subunit at 0.43 mM, of mutation P52L, large subunit, plus mutation P308L, small subunit at 2.5 mM, of mutation P52L, large subunit, plus mutation R350K, small subunit at 1.9 mM Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 alpha-D-glucose 1-phosphate wild-type, 37°C, pH 7.0 Solanum tuberosum
0.17
-
 ATP wild-type, 37°C, pH 7.0 Solanum tuberosum
0.21
-
 alpha-D-glucose 1-phosphate mutation P52L, large subunit, plus mutation P112L, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.22
-
 ATP mutation P52L, large subunit, plus mutation P112L, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.24
-
 ATP mutation P52L, large subunit, plus mutation L46F, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.24
-
 ATP mutation P52L, large subunit, plus mutation P308L, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.25
-
 ATP mutation P52L, large subunit, 37°C, pH 7.0 Solanum tuberosum
0.29
-
 ATP mutation P52L, large subunit, plus mutation R350K, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.36
-
 alpha-D-glucose 1-phosphate mutation P52L, large subunit, plus mutation L46F, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.39
-
 alpha-D-glucose 1-phosphate mutation P52L, large subunit, plus mutation P308L, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.41
-
 alpha-D-glucose 1-phosphate mutation P52L, large subunit, plus mutation R350K, small subunit, 37°C, pH 7.0 Solanum tuberosum
0.42
-
 alpha-D-glucose 1-phosphate mutation P52L, large subunit, 37°C, pH 7.0 Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum ADP-D-glucose + diphosphate
-
 r
diphosphate + ADP
-
 Solanum tuberosum ATP + alpha-D-glucose
-
 r