Literature summary for 2.7.7.27 extracted from

Gomez Casati, D.F.; Aon, M.A.; Iglesias, A.A.
Kinetic and structural analysis of the ultrasensitive behaviour of cyanobacterial ADP-glucose pyrophosphorylase (2000), Biochem. J., 350, 139-147.

No PubMed abstract available

Search for more literature for 2.7.7.27

Activating Compound

Activating Compound	Comment	Organism	Structure
3-phosphoglycerate	the polyethylene glycerol induced molecular crowding increases the sensitivity of enzyme to the cross-talk between 3-phosphoglycerate and phosphate, the ultrasensitive behaviour is correlated with intramolecular conformational changes induced in the tertiary structure of the homotetrameric enzyme	Anabaena sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli AC70R1-504	Anabaena sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphate	the inhibitory action of phosphate interferes with the activating role exerted by 3-phosphoglycerate, a 2fold higher positive cooperation is induced by allosteric inhibitor in crowded medium in comparison to the aqueous condition	Anabaena sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	comparison of Km of alpha-D-glucose 1-phosphate at different ATP concentrations, at two levels of 3-phosphoglycerate and in aqueous or by polyethylenglycol crowded medium, comparison of Km of ATP at different alpha-D-glucose 1-phosphate concentrations, at two levels of 3-phosphoglycerate and in aqueous or by polyethylenglycol crowded medium	Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
200000	-	gel filtration	Anabaena sp.

Organism

Organism	UniProt	Comment	Textmining
Anabaena sp.	-	PCC 7120, cyanobacterium	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Anabaena sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + alpha-D-glucose 1-phosphate	-	Anabaena sp.	diphosphate + ADP-glucose	-	?

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Release 2023.1 (January 2023)