Literature summary for 2.7.7.27 extracted from

Ballicora, M.A.; Laughlin, M.J.; Fu, Y.; Okita, T.W.; Barry, G.F.; Preiss, J.
Adenosine 5'-diphosphate-glucose pyrophosphorylase from potato tuber. Significance of the N terminus of the small subunit for catalytic properties and heat stability (1995), Plant Physiol., 109, 245-251.

Search for more literature for 2.7.7.27

Activating Compound

Activating Compound	Comment	Organism	Structure
3-phosphoglycerate	the homotetrameric small subunit has much lower affinity for activator than the heterotetrameric enzyme	Solanum tuberosum

Cloned(Commentary)

Cloned (Comment)	Organism
The genes of both subunits of enzyme are cloned, expression in Escherichia coli strain AC70R1-504, the one cloned small subunit lacks 10 amino acids of the N-terminus, and is defined as the truncated small subunit. A new cDNA clone of the small subunit encodes a polypeptide that has 10 amino acids more at the N-terminus than the truncated small subunits	Solanum tuberosum

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphate	-	Solanum tuberosum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	alpha-D-glucose 1-phosphate	pH 8.0, 37°C, recombinant enzyme	Solanum tuberosum
0.14	-	alpha-D-glucose 1-phosphate	pH 8.0, 37°C	Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	4 * 50000, the small subunit alone, SDS-PAGE	Solanum tuberosum

Organism

Organism	UniProt	Comment	Textmining
Solanum tuberosum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
the recombinant enzyme containing the large subunit and the truncated small subunit, the recombinant enzyme containing the small subunit and the large subunit and the small subunit alone	Solanum tuberosum

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-alpha-D-glucose	the small and large subunits of the enzyme define the catalytic and regulatory subunits	Solanum tuberosum	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
tuber	-	Solanum tuberosum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	the small subunit alone is highly active but the large subunit alone has negligible catalytic activity	Solanum tuberosum
20	-	final preparation of the small subunits	Solanum tuberosum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + alpha-D-glucose 1-phosphate	-	Solanum tuberosum	diphosphate + ADP-glucose	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	the large subunit is 51000 Da, major function is to modulate the regulatory properties of the enzyme and the small subunit is 50000 Da, major function is catalysis, SDS-PAGE	Solanum tuberosum
homotetramer	4 * 50000, the small subunit alone, SDS-PAGE	Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	large and truncated small subunits: 24% remaining activity, large and small subunits: 89% remaining activity, the small subunit: 98% remainig activity after heat treatment	Solanum tuberosum
65	-	large and truncated small subunits: 1.4% remaining activity, large and small subunits: 83% remaining activity, the small subunit: 102% remainig activity after heat treatment	Solanum tuberosum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.04	-	phosphate	pH 8.0, 37°C, recombinant heterotetrameric enzyme, in absence of 3-phosphoglycerate	Solanum tuberosum
0.08	-	phosphate	pH 8.0, 37°C, the small subunit of enzyme, in presence of 3 mM 3-phosphoglycerate	Solanum tuberosum
0.63	-	phosphate	pH 8.0, 37°C, recombinant heterotetrameric enzyme, in presence of 3 mM 3-phosphoglycerate	Solanum tuberosum

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Release 2023.1 (January 2023)