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Literature summary for 2.7.7.27 extracted from

  • Ghosh, H.P.; Preiss, J.
    Adenosine diphosphate glucose pyrophosphorylase. A regulatory enzyme in the biosynthesis of starch in spinach leaf chloroplasts (1966), J. Biol. Chem., 241, 4491-4504.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
2,3-diphosphoglycerate activation Spinacia oleracea
2,3-diphosphoglycerate actvation of ADP-glucose synthesis Spinacia oleracea
3-phosphoglycerate allosteric activation Spinacia oleracea
3-phosphoglycerate activation Spinacia oleracea
3-phosphoglycerate pH-dependent Spinacia oleracea
D-fructose 1,6-bisphosphate kinetics Spinacia oleracea
D-fructose 1,6-bisphosphate activation of pyrophosphorolysis Spinacia oleracea
D-fructose 1,6-bisphosphate activation of ADP-glucose synthesis Spinacia oleracea
D-fructose 6-phosphate activation Spinacia oleracea
D-fructose 6-phosphate activation of ADPglucose synthesis Spinacia oleracea
D-ribose 5-phosphate activation Spinacia oleracea
D-ribose 5-phosphate slight activation Spinacia oleracea
D-ribose 5-phosphate activation of ADP-glucose synthesis Spinacia oleracea
phosphoenolpyruvate activation Spinacia oleracea
phosphoenolpyruvate activation of pyrophosphorolysis Spinacia oleracea
phosphoenolpyruvate ADPglucose synthesis Spinacia oleracea
Phosphoglycolate slight activation, ADP-glucose synthesis Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Spinacia oleracea
phosphate 3-phosphoglycerate reverses, increasing concentration of Mg2+ does not overcome the inhibition; kinetics Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Spinacia oleracea
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Spinacia oleracea one of the main regulatory steps in starch biosynthesis in plants diphosphate + ADP-glucose
-
 r

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Spinacia oleracea diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate one of the main regulatory steps in starch biosynthesis in plants Spinacia oleracea diphosphate + ADP-glucose
-
 r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.038
-
 phosphate pH 8.5, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
0.06
-
 phosphate pH 7.5, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
0.14
-
 phosphate pH 7.0, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
0.72
-
 phosphate pH 8.5, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
1.15
-
 phosphate pH 7.5, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
1.2
-
 ADP pH 7.5, 37°C, in absence of 3-phosphoglycerate Spinacia oleracea
1.5
-
 phosphate pH 7.0, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea
2
-
 ADP pH 7.5, 37°C, in presence of 3-phosphoglycerate Spinacia oleracea