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Literature summary for 2.7.7.24 extracted from

  • Bais, V.; Batra, S.; Prakash, B.
    Identification of two highly promiscuous thermostable sugar nucleotidylyltransferases for glycorandomization (2018), FEBS J., 285, 2840-2855 .
    View publication on PubMed
Search for more literature for 2.7.7.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.102
-
 UTP pH 7.6, 37°C Pyrococcus horikoshii
0.12
-
 dTTP pH 7.6, 37°C Pyrococcus horikoshii
0.184
-
 GTP pH 7.6, 37°C Pyrococcus horikoshii
0.226
-
 alpha-D-glucose 1-phosphate cosubstrate UTP, pH 7.6, 37°C Pyrococcus horikoshii
0.276
-
 CTP pH 7.6, 70°C Pyrococcus horikoshii
0.299
-
 GTP pH 7.6, 70°C Pyrococcus horikoshii
0.336
-
 ATP pH 7.6, 70°C Pyrococcus horikoshii
0.341
-
 UTP pH 7.6, 70°C Pyrococcus horikoshii
0.366
-
 dTTP pH 7.6, 70°C Pyrococcus horikoshii
0.429
-
 CTP pH 7.6, 37°C Pyrococcus horikoshii
0.68
-
 ATP pH 7.6, 37°C Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Storage Stability

Storage Stability Organism
4°C, stable for weeks Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Pyrococcus horikoshii diphosphate + ADP-alpha-D-glucose
-
 ?
CTP + alpha-D-glucose 1-phosphate
-
 Pyrococcus horikoshii diphosphate + CDP-alpha-D-glucose
-
 ?
dTTP + alpha-D-glucose 1-phosphate
-
 Pyrococcus horikoshii diphosphate + dTDP-alpha-D-glucose
-
 ?
GTP + alpha-D-glucose 1-phosphate
-
 Pyrococcus horikoshii diphosphate + GDP-alpha-D-glucose
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Pyrococcus horikoshii diphosphate + UDP-alpha-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
glucose-1-phosphate thymidylyltransferase
-
 Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 CTP pH 7.6, 37°C Pyrococcus horikoshii
0.36
-
 GTP pH 7.6, 37°C Pyrococcus horikoshii
0.37
-
 dTTP pH 7.6, 37°C Pyrococcus horikoshii
0.4
-
 ATP pH 7.6, 37°C Pyrococcus horikoshii
1.8
-
 ATP pH 7.6, 70°C Pyrococcus horikoshii
1.8
-
 CTP pH 7.6, 70°C Pyrococcus horikoshii
2
-
 dTTP pH 7.6, 70°C Pyrococcus horikoshii
2.1
-
 UTP pH 7.6, 37°C Pyrococcus horikoshii
2.5
-
 GTP pH 7.6, 70°C Pyrococcus horikoshii
6.18
-
 alpha-D-glucose 1-phosphate cosubstrate UTP, pH 7.6, 37°C Pyrococcus horikoshii
12.1
-
 UTP pH 7.6, 70°C Pyrococcus horikoshii