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Literature summary for 2.7.7.23 extracted from

  • Zhang, Z.; Akutsu, J.; Tsujimura, M.; Kawarabayasi, Y.
    Increasing in archaeal GlcNAc-1-P uridyltransferase activity by targeted mutagenesis while retaining its extreme thermostability (2007), J. Biochem., 141, 553-562 .
    View publication on PubMed
Search for more literature for 2.7.7.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
D208A GlcNAc-1-P UTase activity is 10% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
D99A no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
E146A no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
G9A GlcNAc-1-P UTase activity is less than 5% Sulfurisphaera tokodaii
K147A GlcNAc-1-P UTase activity is 25% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
K23A no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
R13A GlcNAc-1-P UTase activity is 10% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
T80A GlcNAc-1-P UTase activity is 56% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
T80L no GlcNAc-1-P UTase activity Sulfurisphaera tokodaii
Y97A GlcNAc-1-P UTase activity is 66% of the activity of wild-type ST0452 protein Sulfurisphaera tokodaii
Y97F GlcNAc-1-P UTase activity is similar to wild-type ST0452 protein Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 UTP pH 7.5, 80°C Sulfurisphaera tokodaii
0.008
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
0.016
-
 diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
0.016
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Co2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Mg2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Mn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii
Zn2+ no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+ Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 Sulfurisphaera tokodaii UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 Sulfurisphaera tokodaii DSM 16993 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 r
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 r

Synonyms

Synonyms Comment Organism
GlcNAc-1-P uridyltransferase
-
 Sulfurisphaera tokodaii
GlcNAc-1-P UTase
-
 Sulfurisphaera tokodaii
ST0452
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.54
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
3.53
-
 UTP pH 7.5, 80°C Sulfurisphaera tokodaii
6.21
-
 diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
8.4
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
320
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
380
-
 diphosphate pH 7.5, 80°C Sulfurisphaera tokodaii
530
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C Sulfurisphaera tokodaii
2120
-
 UTP pH 7.5, 80°C Sulfurisphaera tokodaii