BRENDA - Enzyme Database
show all sequences of 2.7.7.23

Molecular and functional analysis of UDP-N-acetylglucosamine pyrophosphorylases from the migratory locust, Locusta migratoria

Liu, X.; Li, F.; Li, D.; Ma, E.; Zhang, W.; Zhu, K.Y.; Zhang, J.; PLoS ONE 8, e71970 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene LmUAP1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis; gene LmUAP2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Locusta migratoria
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
the enzyme sequence cotains no signal peptide and transmembrane regions; the enzyme sequence cotains no signal peptide and transmembrane regions
Locusta migratoria
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55100
-
-
Locusta migratoria
55800
-
-
Locusta migratoria
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
Locusta migratoria
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Locusta migratoria
T1RR64
-
-
Locusta migratoria
T1RRG3
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
developmental expression pattern of LmUAP1, overview. The expression of LmUAP1 is significantly higher during the early embryogenesis (days 2-4) than the rest of the embryogenetic stages, highest expression is found just after fourth-instar nymph molting, but the expression gradually declined; developmental expression pattern of LmUAP2, overview. The expression of LmUAP2 is relatively consistent during the embryogenesis except for the 5-day-old eggs, in which the expression is 1.5 to 3fold higher than those in other days. The expression level of LmUAP2 is relatively consistent during the stages of after fourth-instar nymph molting and fifth-instar nymphs molted to adults; widely expressed in all the major tissues besides chitin-containing tissues and consistently expressed throughout early embryogenesis, egg hatch and nymphal development; widely expressed in all the major tissues besides chitin-containing tissues. High expression of UAP1 is detected during early embryogenesis, then decreases greatly, and slowly increases before egg hatch. During nymphal development, the highest expression of LmUAP1 appears just after molting but declines in each intermolting period and then increases before molting to the next stage
Locusta migratoria
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
739475
Locusta migratoria
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55100, approximately, sequence calculation; x * 55800, approximately, sequence calculation
Locusta migratoria
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Locusta migratoria
sequence calculation
-
5.64
Locusta migratoria
sequence calculation
-
5.74
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LmUAP1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Locusta migratoria
gene LmUAP2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
Locusta migratoria
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
the enzyme sequence cotains no signal peptide and transmembrane regions
Locusta migratoria
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55100
-
-
Locusta migratoria
55800
-
-
Locusta migratoria
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
Locusta migratoria
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
developmental expression pattern of LmUAP1, overview. The expression of LmUAP1 is significantly higher during the early embryogenesis (days 2-4) than the rest of the embryogenetic stages, highest expression is found just after fourth-instar nymph molting, but the expression gradually declined; widely expressed in all the major tissues besides chitin-containing tissues. High expression of UAP1 is detected during early embryogenesis, then decreases greatly, and slowly increases before egg hatch. During nymphal development, the highest expression of LmUAP1 appears just after molting but declines in each intermolting period and then increases before molting to the next stage
Locusta migratoria
-
additional information
developmental expression pattern of LmUAP2, overview. The expression of LmUAP2 is relatively consistent during the embryogenesis except for the 5-day-old eggs, in which the expression is 1.5 to 3fold higher than those in other days. The expression level of LmUAP2 is relatively consistent during the stages of after fourth-instar nymph molting and fifth-instar nymphs molted to adults; widely expressed in all the major tissues besides chitin-containing tissues and consistently expressed throughout early embryogenesis, egg hatch and nymphal development
Locusta migratoria
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
739475
Locusta migratoria
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55100, approximately, sequence calculation
Locusta migratoria
?
x * 55800, approximately, sequence calculation
Locusta migratoria
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Locusta migratoria
sequence calculation
-
5.64
Locusta migratoria
sequence calculation
-
5.74
General Information
General Information
Commentary
Organism
evolution
UAP isozymes are encoded by two different genes, LmUAP1 and LmUAP2; UAP isozymes are encoded by two different genes, LmUAP1 and LmUAP2
Locusta migratoria
physiological function
injection of early second- and fifth-instar nymphs (1-day-old) or middle second- and fifth-instar nymphs (3- to 4-day-old) with UAP2 RNAi does not interfere with normal development and molting of insects; injection of early second- and fifth-instar nymphs (1-day-old) with UAP1 RNAi results in 100% mortality 2 days after the injection. Injection of middle second- and fifth-instar nymphs (3- to 4-day-old) with UAP1 RNAi results in 100% mortality during their next molting process; the enzyme functions in the formation of extracellular matrix by producing N-acetylglucosamine (GlcNAc) residues needed for chitin biosynthesis and protein glycosylation; the enzyme functions in the formation of extracellular matrix by producing N-acetylglucosamine (GlcNAc) residues needed for chitin biosynthesis and protein glycosylation
Locusta migratoria
General Information (protein specific)
General Information
Commentary
Organism
evolution
UAP isozymes are encoded by two different genes, LmUAP1 and LmUAP2
Locusta migratoria
physiological function
injection of early second- and fifth-instar nymphs (1-day-old) with UAP1 RNAi results in 100% mortality 2 days after the injection. Injection of middle second- and fifth-instar nymphs (3- to 4-day-old) with UAP1 RNAi results in 100% mortality during their next molting process; the enzyme functions in the formation of extracellular matrix by producing N-acetylglucosamine (GlcNAc) residues needed for chitin biosynthesis and protein glycosylation
Locusta migratoria
physiological function
injection of early second- and fifth-instar nymphs (1-day-old) or middle second- and fifth-instar nymphs (3- to 4-day-old) with UAP2 RNAi does not interfere with normal development and molting of insects; the enzyme functions in the formation of extracellular matrix by producing N-acetylglucosamine (GlcNAc) residues needed for chitin biosynthesis and protein glycosylation
Locusta migratoria
Other publictions for EC 2.7.7.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746844
Honda
Improvement of ST0452 N-acety ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
Appl. Environ. Microbiol.
84
e002213-18
2018
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1
1
2
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10
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1
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-
748113
Dadashipour
Identification of a direct bi ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
J. Bacteriol.
200
e00048-18
2018
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-
-
-
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2
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8
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4
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4
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1
1
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746841
Honda
Increasing the thermostable s ...
Escherichia coli, Escherichia coli K12, Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
Appl. Environ. Microbiol.
83
e02291
2017
-
-
-
-
15
-
-
4
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-
-
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12
-
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2
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-
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4
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2
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4
2
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15
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4
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2
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4
-
2
-
-
4
2
-
-
-
-
-
-
-
4
4
736517
Sharma
Escherichia coli N-acetylgluco ...
Escherichia coli, Escherichia coli ATCC 25922
J. Biomol. Screen.
21
342-353
2016
-
-
1
-
-
-
1
-
-
1
-
2
-
4
-
-
1
-
-
-
-
-
2
-
1
1
-
-
1
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-
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1
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1
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1
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2
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1
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-
2
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1
1
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-
1
-
-
-
-
-
-
-
-
-
737096
Dziadek
Mycobacterium tuberculosis Ats ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
PLoS ONE
11
e0148030
2016
-
-
1
-
1
-
-
-
-
-
-
3
-
9
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1
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6
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1
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1
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3
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1
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6
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-
1
1
-
-
-
738383
Shi
Two Leptinotarsa uridine dipho ...
Leptinotarsa decemlineata
Insect Biochem. Mol. Biol.
68
1-12
2016
-
-
1
-
1
-
-
-
-
-
-
2
-
2
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12
-
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2
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2
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2
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2
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24
-
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2
-
-
-
-
-
-
-
-
-
2
2
4
4
-
-
729696
Zhang
Characterization of the amino ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7
Extremophiles
19
417-427
2015
-
-
1
-
6
-
-
-
-
1
-
3
-
4
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-
-
-
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-
3
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6
2
1
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1
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1
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6
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1
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3
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-
3
-
6
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
735518
Sharma
Identification and characteriz ...
Escherichia coli, Escherichia coli ATCC 25922
Appl. Microbiol. Biotechnol.
100
3071-3085
2015
-
-
1
-
-
-
-
-
-
1
-
4
-
4
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1
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4
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1
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1
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1
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1
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4
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1
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4
-
1
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1
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-
-
-
1
1
-
-
-
735569
Patin
Purification and biochemical c ...
Yersinia pseudotuberculosis, Yersinia pseudotuberculosis YPIII
Arch. Microbiol.
197
371-378
2015
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1
-
1
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2
-
1
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2
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2
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1
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2
2
1
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2
1
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1
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1
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1
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2
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1
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2
2
1
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2
1
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-
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-
2
2
-
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737150
Soni
Depletion of M. tuberculosis G ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
PLoS Pathog.
11
e1005235
2015
-
1
1
-
-
-
1
-
-
-
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6
-
13
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6
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1
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1
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1
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1
1
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1
1
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6
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6
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1
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1
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-
2
2
-
-
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737291
Rani
High-throughput screen identif ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Tuberculosis
95
664-677
2015
-
-
1
-
-
-
2
3
-
1
-
4
-
9
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1
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4
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1
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1
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1
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1
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1
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4
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1
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4
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1
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1
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2
2
-
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737391
Edwards
Structure of uridine diphospha ...
Entamoeba histolytica
Acta Crystallogr. Sect. F
71
560-565
2015
-
1
1
1
-
-
-
-
-
-
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2
-
2
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1
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2
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2
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2
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2
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3
6
-
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738765
Wang
Functional inactivation of UDP ...
Oryza sativa Japonica Group, Oryza sativa Japonica Group ZH11
J. Exp. Bot.
66
973-987
2015
-
-
1
-
1
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4
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4
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3
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4
1
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1
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1
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3
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4
1
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2
2
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740262
Wang
Probing the roles of conserved ...
Escherichia coli
Carbohydr. Res.
413
70-74
2015
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1
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15
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1
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1
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1
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3
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1
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1
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15
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1
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3
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1
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1
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3
3
-
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741100
Qi
Novel lead compound optimizati ...
Xanthomonas oryzae pv. oryzae
Pestic. Biochem. Physiol.
122
22-28
2015
-
1
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-
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13
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1
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1
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1
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1
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1
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1
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12
13
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1
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1
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1
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1
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1
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-
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735396
Vithani
GlmU (N-acetylglucosamine-1-ph ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. F
70
703-708
2014
-
-
-
1
-
-
2
-
-
1
-
1
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1
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2
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1
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1
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1
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1
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1
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2
2
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722022
Santos Junior
Structure-based drug design st ...
Moniliophthora perniciosa
Chem. Cent. J.
7
48
2013
-
1
-
1
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-
13
-
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-
1
-
4
-
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2
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1
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1
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13
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1
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2
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2
2
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737319
Urbaniak
A novel allosteric inhibitor o ...
Homo sapiens, Trypanosoma brucei, Trypanosoma brucei brucei, Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
ACS Chem. Biol.
8
1981-1987
2013
-
1
3
2
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8
1
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1
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1
-
6
-
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1
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1
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4
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1
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3
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1
3
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2
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3
8
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1
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1
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1
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1
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3
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643071
Bulik
UDP-N-acetylglucosamine pyroph ...
Giardia intestinalis
J. Biol. Chem.
275
14722-14728
2000
1
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2
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3
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643077
Brown
Crystal structure of the bifun ...
Escherichia coli
EMBO J.
18
4096-4107
1999
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-
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2
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2
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643067
Bulik
Purification and characterizat ...
Giardia intestinalis
Mol. Biochem. Parasitol.
95
135-139
1998
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1
4
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4
2
2
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2
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2
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1
1
5
1
1
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1
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643068
Mio
The eukaryotic UDP-N-acetylglu ...
Candida albicans, Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
273
14392-14397
1998
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3
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9
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9
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7
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7
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3
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1
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12
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9
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3
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9
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9
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7
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3
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1
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12
-
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9
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643069
Wang-Gillam
A 17-amino acid insert changes ...
Homo sapiens, Sus scrofa
J. Biol. Chem.
273
27055-27057
1998
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1
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2
2
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4
2
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2
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1
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3
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6
1
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1
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2
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2
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1
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3
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6
1
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643065
Szumilo
Purification to homogeneity an ...
Sus scrofa
J. Biol. Chem.
271
13147-13154
1996
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-
-
-
-
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7
1
4
2
2
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1
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1
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8
1
2
5
1
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1
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1
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7
1
4
2
2
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1
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8
1
2
5
1
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1
-
1
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-
643066
De Luca
Overexpression, one-step purif ...
Escherichia coli
Bioorg. Med. Chem.
4
131-141
1996
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1
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1
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1
3
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2
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1
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1
2
4
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1
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1
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1
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3
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1
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1
2
4
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1
1
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643064
Ullrich
Identification of the gonococc ...
Neisseria gonorrhoeae
J. Bacteriol.
177
6902-6909
1995
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1
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2
1
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4
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2
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1
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1
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2
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-
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-
288685
Mengin-Lecreulx
Copurification of glucosamine- ...
Escherichia coli
J. Bacteriol.
176
5788-5795
1994
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1
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1
1
2
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1
1
3
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1
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1
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1
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5
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1
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1
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1
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1
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1
1
3
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1
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1
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5
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1
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-
643062
Yamamoto
Inhibition of UDP-N-acetylgluc ...
Neurospora crassa, Saccharomyces cerevisiae
Biochim. Biophys. Acta
614
367-372
1980
-
-
-
-
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8
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4
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6
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6
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8
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6
-
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643061
Yamamoto
Purification and some properti ...
Neurospora crassa, Neurospora crassa IFO 6178
Can. J. Microbiol.
25
1381-1386
1979
4
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1
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2
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3
1
4
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3
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1
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1
2
6
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1
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1
1
1
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4
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1
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2
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3
1
4
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1
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1
2
6
-
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1
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1
1
1
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643060
Yamamoto
-
The effect of magnesium ion on ...
Saccharomyces cerevisiae
J. Ferment. Technol.
56
57-58
1978
1
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2
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1
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1
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1
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1
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1
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2
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643059
Yamamoto
-
Purification and charcterizati ...
Saccharomyces cerevisiae
Agric. Biol. Chem.
40
2275-2281
1976
2
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1
4
2
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3
2
2
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1
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1
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1
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3
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1
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1
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2
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1
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4
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2
2
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1
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1
2
3
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1
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1
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643057
Pattabiraman
Purification of uridine diphos ...
Ovis aries
Biochim. Biophys. Acta
50
129-134
1961
-
-
-
-
-
1
3
2
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4
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1
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2
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1
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2
1
2
2
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1
1
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1
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4
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1
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1
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2
1
2
2
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1
1
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643058
Strominger
Uridine diphosphoacetylglucosa ...
Bos taurus, Staphylococcus aureus
J. Biol. Chem.
234
1822-1827
1959
-
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-
-
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2
1
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3
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2
1
2
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2
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1
2
4
6
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2
1
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2
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1
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3
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2
1
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2
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1
2
4
6
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2
1
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