Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
generation of a three-dimensional model, using human GlcNAc1p nucleotidyltransferase complexed with UDP-GlcNAc, PDB code 1JV1 | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 0.5 mM, 56% reduction in activity | Arabidopsis thaliana | |
EDTA | 5 mM, 11% residual activity | Arabidopsis thaliana | |
hygromycin | 0.5 mg/ml, 36% reduction in activity | Arabidopsis thaliana | |
streptomycin | 0.5 mg/ml, 74% reduction in activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
0.18 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
0.203 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
0.219 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
0.296 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
0.337 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
0.808 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
2.768 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, required | Arabidopsis thaliana | |
Mn2+ | or Mg2+, required | Arabidopsis thaliana | |
Mn2+ | or Mg2+, required. At 5 mM, 126% of the activity with Mg2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58300 | - |
1 * 58300, SDS-PAGE | Arabidopsis thaliana |
60000 | - |
gel filtration | Arabidopsis thaliana |
60000 | - |
x * 60000, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O64765 | isoform GLCNAC1PUT2 | - |
Arabidopsis thaliana | Q940S3 | isoform GLCNAC1PUT1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme additionally shows the activity of UTP-glucose-1-phosphate uridylyltransferase, EC 2.7.7.9 | Arabidopsis thaliana | ? | - |
? | |
additional information | no substrates: CTP, GTP, ITP or ATP, glucose 1-phosphate, glucosamine 1-phosphate, xylose 1-phosphate, galactose 1-phosphate, galactosamin 1-phosphate, fucose 1-phosphate, mannose 1-phosphate, galactosamine 1-phosphate, fructose 1-phosphate, or glucose 6-phosphate | Arabidopsis thaliana | ? | - |
? | |
N-acetyl-D-glucosamine 1-phosphate + UTP | - |
Arabidopsis thaliana | UDP-N-acetyl-D-glucosamine + diphosphate | - |
r | |
UDP-N-acetyl-D-galactosamine + diphosphate | - |
Arabidopsis thaliana | N-acetyl-D-galactosamine 1-phosphate + UTP | - |
r | |
UDP-N-acetyl-D-glucosamine + diphosphate | - |
Arabidopsis thaliana | N-acetyl-D-glucosamine 1-phosphate + UTP | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Arabidopsis thaliana |
monomer | 1 * 58300, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GLCNAC1PUT1 | - |
Arabidopsis thaliana |
GLCNAC1PUT2 | - |
Arabidopsis thaliana |
UDP-N-acetylglucosamine diphosphorylase 1 | - |
Arabidopsis thaliana |
UDP-N-acetylglucosamine diphosphorylase 2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
65 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.3 | 9 | - |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
18 | - |
UDP-N-acetyl-D-galactosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
110 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
120 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
140 | - |
UTP | pH 7.6, 37°C | Arabidopsis thaliana | |
160 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 7.6, 37°C | Arabidopsis thaliana | |
220 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana | |
460 | - |
UDP-N-acetyl-D-glucosamine | pH 7.6, 37°C | Arabidopsis thaliana |