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Literature summary for 2.7.7.23 extracted from

  • Yin, J.; Garen, C.R.; Cherney, M.M.; Cherney, L.T.; James, M.N.
    Expression, purification and preliminary crystallographic analysis of N-acetylglucosamine-1-phosphate uridylyltransferase from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. F, F64, 805-808.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using 20% PEG 3350, 0.15 M DL-malate pH 7.0 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis bifunctional enzyme that catalyzes two consecutive reactions leading to the biosynthesis of UDP-N-acetylglucosamine, in the first reaction, GlmU catalyzes the CoA-dependent acetylation of glucosamine-1-phosphate to afford N-acetylglucosamine-1-phosphate, in the second reaction, the UMP moiety from a UTP molecule is transferred to N-acetyl-alpha-D-glucosamine 1-phosphate to give the final product UDP-GlcNAc ?
-
 ?
additional information Mycobacterium tuberculosis H37Rv bifunctional enzyme that catalyzes two consecutive reactions leading to the biosynthesis of UDP-N-acetylglucosamine, in the first reaction, GlmU catalyzes the CoA-dependent acetylation of glucosamine-1-phosphate to afford N-acetylglucosamine-1-phosphate, in the second reaction, the UMP moiety from a UTP molecule is transferred to N-acetyl-alpha-D-glucosamine 1-phosphate to give the final product UDP-GlcNAc ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and GSTrap column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine
-
 Mycobacterium tuberculosis UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine
-
 Mycobacterium tuberculosis H37Rv UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
additional information bifunctional enzyme that catalyzes two consecutive reactions leading to the biosynthesis of UDP-N-acetylglucosamine, in the first reaction, GlmU catalyzes the CoA-dependent acetylation of glucosamine-1-phosphate to afford N-acetylglucosamine-1-phosphate, in the second reaction, the UMP moiety from a UTP molecule is transferred to N-acetyl-alpha-D-glucosamine 1-phosphate to give the final product UDP-GlcNAc Mycobacterium tuberculosis ?
-
 ?
additional information bifunctional enzyme that catalyzes two consecutive reactions leading to the biosynthesis of UDP-N-acetylglucosamine, in the first reaction, GlmU catalyzes the CoA-dependent acetylation of glucosamine-1-phosphate to afford N-acetylglucosamine-1-phosphate, in the second reaction, the UMP moiety from a UTP molecule is transferred to N-acetyl-alpha-D-glucosamine 1-phosphate to give the final product UDP-GlcNAc Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

Subunits Comment Organism
trimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
GlmU
-
 Mycobacterium tuberculosis
N-acetylglucosamine-1-phosphate uridylyltransferase
-
 Mycobacterium tuberculosis