Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells as a glutathione S-transferase fusion protein | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Candida albicans | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Candida albicans | |
Ca2+ | Ca2+ shows similar enhancement like Mg2+ | Candida albicans | |
Mg2+ | - |
Candida albicans | |
Mg2+ | Mg2+ enhances the enzymatic activity by increasing the affinity between UTP and the enzyme | Candida albicans | |
Zn2+ | - |
Candida albicans | |
Zn2+ | Zn2+ shows similar enhancement like Mg2+ | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54700 | - |
- |
Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate | Candida albicans | - |
uridine-diphospho-N-acetylglucosamine + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography and Superdex 75 gel filtration | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | the reaction proceeds as an SN2 reaction | Candida albicans | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate | - |
Candida albicans | uridine-diphospho-N-acetylglucosamine + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GlmU | - |
Candida albicans |
UAP enzyme | - |
Candida albicans |
UAP1 | - |
Candida albicans |
UDP-GlcNAc pyrophosphorylase | - |
Candida albicans |
uridine-diphospho-N-acetylglucosamine pyrophosphorylase | - |
Candida albicans |