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Literature summary for 2.7.7.23 extracted from

  • Szumilo, T.; Zeng, Y.; Pastuszak, I.; Drake, R.; Szumilo, H.; Elbein, A.D.
    Purification to homogeneity and properties of UDP-GlcNAc (GalNAc) pyrophosphorylase (1996), J. Biol. Chem., 271, 13147-13154.
    View publication on PubMed
Search for more literature for 2.7.7.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 UDP-N-acetyl-D-glucosamine pH 8.5, 37°C, 5 mM diphosphate Sus scrofa
0.29
-
 N-acetyl-D-glucosamine 1-phosphate pH 7.5, 37°C, 5 mM UTP Sus scrofa
0.42
-
 UTP pH 7.5, 37°C, 10 mM N-acetyl-D-glucose 1-phosphate Sus scrofa
1.1
-
 N-acetyl-alpha-D-galactosamine 1-phosphate pH 7.5, 37°C, 5 mM UTP Sus scrofa
2.2
-
 UDP-N-acetyl-D-galactosamine pH 8.5, 37°C, 5 mM diphosphate Sus scrofa
5.8
-
 D-glucose 1-phosphate pH 7.5, 37°C, 5 mM UTP Sus scrofa
6.3
-
 UDP-D-glucose pH 8.5, 37°C, 5 mM diphosphate Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mn2+ Sus scrofa
Mg2+ can partially replace Mn2+ Sus scrofa
Mn2+ required for maximum activity, 0.5-1 mM Sus scrofa
Ni2+ can partially replace Mn2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 SDS-PAGE, degradation products at 57000 and 49000 Da Sus scrofa
125000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine Sus scrofa amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine Sus scrofa involved in formation of N-linked oligosaccharides UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
 Sus scrofa
-
brain
-
 Sus scrofa
-
heart
-
 Sus scrofa
-
kidney
-
 Sus scrofa
-
liver
-
 Sus scrofa
-
lung
-
 Sus scrofa
-
pancreas very high activity Sus scrofa
-
spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.9
-
 purified enzyme Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM 2-mercaptoethanol, 4 months, 10% loss of activity Sus scrofa
0°C, 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.05% NaN3, 5 months, 50% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-glucose
-
 Sus scrofa UTP + D-glucose 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-galactosamine
-
 Sus scrofa UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine
-
 Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine involved in formation of N-linked oligosaccharides Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 Sus scrofa diphosphate + UDP-N-acetyl-D-galactosamine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE, gel filtration Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 UTP formation Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 most stable Sus scrofa