Literature summary for 2.7.7.2 extracted from

Leone, P.; Galluccio, M.; Barbiroli, A.; Eberini, I.; Tolomeo, M.; Vrenna, F.; Gianazza, E.; Iametti, S.; Bonomi, F.; Indiveri, C.; Barile, M.
Bacterial production, characterization and protein modeling of a novel monofuctional isoform of FAD synthase in humans An emergency protein? (2018), Molecules, 23, 116 .

Search for more literature for 2.7.7.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of structure	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Hg2+	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00013	-	FMN	pH 7.5, 37°C	Homo sapiens
0.00691	-	ATP	pH 7.5, 37°C	Homo sapiens
0.042	-	diphosphate	pH 7.5, 37°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required, 50% of maximum activity at 0.15 mM	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36500	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8NFF5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + FMN	-	Homo sapiens	diphosphate + FAD	-	r
diphosphate + FAD	-	Homo sapiens	ATP + FMN	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 38266, calculated from sequence, 1 * 36000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
FADS-6	-	Homo sapiens
FLAD1	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
51.6	-	Tm value	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00013	-	FMN	pH 7.5, 37°C	Homo sapiens
0.00691	-	ATP	pH 7.5, 37°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	ration of FAD/monomer is 0.6	Homo sapiens

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Release 2023.1 (January 2023)