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Literature summary for 2.7.7.2 extracted from

  • Liu, S.; Diao, N.; Wang, Z.; Lu, W.; Tang, Y.J.; Chen, T.
    Modular engineering of the flavin pathway in Escherichia coli for improved flavin mononucleotide and flavin adenine dinucleotide production (2019), J. Agric. Food Chem., 67, 6532-6540 .
    View publication on PubMed

Application

Application Comment Organism
synthesis modular engineering of Escherichia coli to improve flavin production and the conversion ratio of riboflavin RF to FMN/FAD. The RF operon and the bifunctional RF kinase/FAD synthetase are divided into two separate modules and expressed at different levels to produce different RF:ribF. The best strain respectively produces 324.1 and 171.6 mg/l of FAD and FMN in shake flask fermentation. Error-prone of the RibF gene further increase production up to 586.1 mg/l in shake flask cultivation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AG40 bifunctional riboflavin kinase/FMN adenylyltransferase, cf. EC 2.7.1.26
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Synonyms

Synonyms Comment Organism
ribF
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Escherichia coli