Cloned (Comment) | Organism |
---|---|
His6-tagged enzyme is expressed in Escherichia coli Rosetta (DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | competitive inhibition | Homo sapiens | |
GTP | uncompetitive inhibition | Homo sapiens | |
Urea | the enzymatic activity of isoform FADS2 decreases dramatically at increasing urea concentration, with a mid-point for activity loss at 2.1 m urea | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
FMN | at 37°C, in 50 mM Tris-HCl, pH 7.5 | Homo sapiens | |
0.0153 | - |
ATP | at 37°C, in 50 mM Tris-HCl, pH 7.5 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ gives maximum FAD synthesis equal to 62% of that measured with Mg2+ | Homo sapiens | |
Co2+ | in the presence of about 0.5 mM Co2+, the activity is almost equal to that measured with Mg2+ | Homo sapiens | |
Mg2+ | MgCl2 is strictly required for both the synthetic and diphosphorolytic activity, FAD synthesis is abolished in the absence of MgCl2 | Homo sapiens | |
Mn2+ | Mn2+ gives maximum FAD synthesis equal to 77% of that measured with Mg2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56537 | - |
x * 56537, isoform FADS2, calculated from amino acid sequence | Homo sapiens |
57000 | - |
x * 57000, isoform FADS2, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NFF5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating column chromatography and hydroxyapatite column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Homo sapiens | diphosphate + FAD | - |
r | |
diphosphate + FAD | low reaction rate | Homo sapiens | ATP + FMN | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 56537, isoform FADS2, calculated from amino acid sequence | Homo sapiens |
? | x * 57000, isoform FADS2, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FADS2 | isoform | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
FMN | at 37°C, in 50 mM Tris-HCl, pH 7.5 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
GTP | at 37°C, in 50 mM Tris-HCl, pH 7.5 | Homo sapiens | |
2.5 | - |
GDP | at 37°C, in 50 mM Tris-HCl, pH 7.5 | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | isoelectric focusing | - |
5.66 |
Homo sapiens | calculated from amino acid sequence | - |
6 |