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Literature summary for 2.7.7.2 extracted from
- Cloning of FAD synthetase gene from Corynebacterium ammoniagenes and its application to FAD and FMN production (1995), Appl. Microbiol. Biotechnol., 42, 724-729.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | industrial production of FAD and FMN as nutrient additives, pharmaceuticals and biochemical agents | Corynebacterium ammoniagenes |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| FAD synthetase overproducing recombinant Corynebacterium ammoniagenes KY13315 constructed from ATCC6872, gene cannot be expressed in Escherichia coli | Corynebacterium ammoniagenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | specific inhibition of adenylyltransferase activity | Corynebacterium ammoniagenes |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | effective for FMN production | Corynebacterium ammoniagenes | |
| Cu2+ | effective for FMN production | Corynebacterium ammoniagenes | |
| additional information | not basically effected by Mg2+, FAD production slightly inhibited at high concentrations | Corynebacterium ammoniagenes | |
| Zn2+ | effective for FMN production | Corynebacterium ammoniagenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | Corynebacterium ammoniagenes | - |
diphosphate + FAD | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium ammoniagenes | - |
ATCC6872 and KY13315, previously Brevibacterium ammoniagenes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | - |
Corynebacterium ammoniagenes | diphosphate + FAD | - |
r | |
| ATP + riboflavin | - |
Corynebacterium ammoniagenes | ? | - |
? | |
| additional information | bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 | Corynebacterium ammoniagenes | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 37 | riboflavin used as substrate | Corynebacterium ammoniagenes |
| 37 | 40 | FMN used as substrate | Corynebacterium ammoniagenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | FMN used as substrate | Corynebacterium ammoniagenes |
| 7.8 | 8 | riboflavin used as substrate | Corynebacterium ammoniagenes |
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