Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta (DE3) pLysS cells | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
mutant C36S/C118V/A152C/C160S/C197S/C257S/C293S/C204V in complex with a chemically modified RNA, to 2.25 A resolution | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
C36S/C118V/A152C/C160S/C197S/C257S/C293S/C204V | introduction of a Cys residue in a mutant lacking all endogenous Cys residues. Mutant achieves maximum specific disulfide cross-linking efficiency. The resulting construct is active and, when mixed with a chemically modified RNA, yields crystals of an enzyme-RNA complex | Bos taurus |
C36S/C118V/C160S/C197S/C257S/C293S/C204V | mutation of all seven endogenous cysteine residues. Mutant is able to bind RNA at a level similar to that of wild-type, but no longer forms nonspecific disulfide cross-links with the modified RNA | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P25500 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Bos taurus |