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Literature summary for 2.7.7.19 extracted from
- Characterization of the RNA binding energetics of the Candida albicans poly(A) polymerase (2007), Yeast, 24, 431-446.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Candida albicans |
General Stability
| General Stability | Organism |
|---|---|
| the protein undergoes structural modifications upon RNA binding, although the interaction does not significantly modify the stability of the protein | Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA | Candida albicans | - |
diphosphate + RNA(A)n | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography and phosphocellulose column chromatography | Candida albicans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + RNAn = diphosphate + RNAn+3'-A | initial interaction between RNA and the enzyme is characterized by a high enthalpy of association. The minimal RNA binding site of the enzyme is eight nucleotides. Upon RNA binding, the enzyme undergoes structural modifications, although the interaction does not significantly modify the stability of the protein | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA | - |
Candida albicans | diphosphate + RNA(A)n | - |
? | |
| additional information | dATP is not used efficiently by the PAP protein, neither CTP nor UTP is an effective substrate, GTP is used at 6.3% of the rate of ATP | Candida albicans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAP | - |
Candida albicans |
| poly(A) polymerase | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
reactions incubated at 50°C result in a decrease of nearly 50% of incorporation | Candida albicans |
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Release 2023.1 (January 2023)
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