General Stability | Organism |
---|---|
freezing and thawing accelerates inactivation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Bos taurus | |
additional information | insensitive to high levels of RNA-polymerase inhibitors | Bos taurus | |
Na2HPO4 | - |
Bos taurus | |
Ribonucleoside triphosphates other than ATP | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | oligoadenylate (in presence of Mn2+) | Bos taurus | |
0.2 | - |
oligoadenylate | in presence of Mg2+, pH 9.0, 35°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bos taurus | 5737 | - |
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
sucrose density gradient sedimentation, gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
calf | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.33 | - |
35°C, pH 9.0 | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 80% inactivation in the first few weeks, the 20% remaining activity is stable for more than 2 years | Bos taurus |
-70°C or under liquid nitrogen, extensive inactivation | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oligoadenylate | - |
Bos taurus | ? | - |
? | |
ATP + RNA | highly specific for ATP | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer: variety of oligoribonucleotides having free 3'-OH | Bos taurus | diphosphate + RNA(A)n | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
ATP | cosubstrates rA(pA)5, pH 9.0, 35°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | about 50% of maximum activity at pH 7.0 and pH 8.8 | Bos taurus |