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Literature summary for 2.7.7.18 extracted from
- Structural and functional characterization of Plasmodium falciparum nicotinic acid nononucleotide adenylyltransferase (2016), J. Mol. Biol., 428, 4946-4961 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structures of NaMNAT in the product-bound state with nicotinic acid adenine dinucleotide and complexed with an alpha,beta-non-hydrolizable ATP analog to a resolution of 2.2 A and 2.5 A, respectively. NaMNAT possesses two cysteine residues within the active site likely to be involved in redox regulation of NaMNAT activity. NaMNAT is capable of utilizing nicotinic acid adenine dinucleotide and nicotinamide mononucleotide, reactions of EC 2.7.7.18 and EC 2.7.7.1, resectively, with a slight preference for nicotinic acid adenine dinucleotide | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C41A | active site mutant, increase in Km values | Plasmodium falciparum |
| C41A/C43A | active site mutant | Plasmodium falciparum |
| C43A | active site mutant, 10fold increase in vmax value | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
nicotinic acid ribonucleotide | mutant C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum |  |
| 0.022 | - |
nicotinic acid ribonucleotide | wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.194 | - |
nicotinic acid ribonucleotide | mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.323 | - |
nicotinic acid ribonucleotide | mutant C41A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.456 | - |
ATP | mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.525 | - |
ATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.624 | - |
ATP | mutant C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 1.186 | - |
ATP | mutant C41A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8IE38 | catalyzes the reactions of both EC 2.7.7.18 and EC 2.7.7.1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicotinic acid ribonucleotide | - |
Plasmodium falciparum | diphosphate + deamido-NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PF3D7_1327600 | - |
Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
ATP | mutant C41A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.065 | - |
nicotinic acid ribonucleotide | wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.082 | - |
ATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.112 | - |
nicotinic acid ribonucleotide | mutant C41A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.7 | - |
ATP | mutant C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 1.033 | - |
nicotinic acid ribonucleotide | mutant C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 1.92 | - |
ATP | mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 2.48 | - |
nicotinic acid ribonucleotide | mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
ATP | mutant C41A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.17 | - |
ATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 0.35 | - |
nicotinic acid ribonucleotide | mutant C41A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 1.17 | - |
ATP | mutant C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 3 | - |
nicotinic acid ribonucleotide | wild-type, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 4.17 | - |
ATP | mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 9.38 | - |
nicotinic acid ribonucleotide | mutant C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
| 12.83 | - |
nicotinic acid ribonucleotide | mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication | Plasmodium falciparum | |
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