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Literature summary for 2.7.7.18 extracted from

  • Konishi, K.; Ueda, S.; Kawano, M.; Osawa, S.; Tamura, T.; Hokazono, E.; Kayamori, Y.; Sakasegawa, S.I.
    Characterization and application of a novel nicotinamide mononucleotide adenylyltransferase from Thermus thermophilus HB8 (2018), J. Biosci. Bioeng., 125, 385-389 .
    View publication on PubMed

Application

Application Comment Organism
analysis NMNAT can be applied to the colorimetric NMN or NaMN assays, which employ either adenylation of NMN to NAD by NMNAT or adenylation of NaMN to deamido-NAD (NaAD) by NMNAT followed by amidation of NaAD to NAD by NAD synthetase (NADS, EC 6.3.1.5) or an NAD cycling reaction using 12alpha-hydroxysteroid dehydrogenase (12a-HSD, EC 1.1.1.176) and diaphorase (DI, EC 1.6.99.3) to accumulate reduced 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium. The enzymatic cycling method enables detection of 0.5 mM (12.2 nM in the reaction mixture) NMN or NaMN in an automatic clinical analyzer Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77800
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SHF0 cf. EC 2.7.7.1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information additionally cataylzes the reaction of EC 2.7.7.1. No donors: ADP, AMP, TMP, GMP, CMP, UMP and IMP Thermus thermophilus ?
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-

Subunits

Subunits Comment Organism
trimer 3 * 22000, SDS-PAGE, x * 21051, calculated from sequence Thermus thermophilus

Synonyms

Synonyms Comment Organism
TTHA1780
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Thermus thermophilus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
70
-
45 min, no loss of activity Thermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermus thermophilus calculated from sequence
-
6.5