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Literature summary for 2.7.7.18 extracted from

  • Wang, X.; Zhou, Y.; Wang, L.; Liu, W.; Liu, Y.; Peng, C.; Zhao, Z.
    Engineering Escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis (2017), Appl. Environ. Microbiol., 83, e00692 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A86W/Y118N increase in activity, and mutant accepts nicotinamide ribonucleotide as substrate Escherichia coli
Y84V/Y118D mutant prefers nicotinamide ribonucleotide over nicotinic acid ribonucleotide bas substrate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30┬░C Escherichia coli
0.11
-
beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30┬░C Escherichia coli
1.7
-
beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30┬░C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140NE60
-
-
Escherichia coli BL21-DE3 A0A140NE60
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-nicotinate D-ribonucleotide
-
Escherichia coli diphosphate + deamido-NAD+
-
?
ATP + beta-nicotinate D-ribonucleotide
-
Escherichia coli BL21-DE3 diphosphate + deamido-NAD+
-
?

Synonyms

Synonyms Comment Organism
nadD
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30┬░C Escherichia coli
4.9
-
beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30┬░C Escherichia coli
25
-
beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30┬░C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.86
-
beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30┬░C Escherichia coli
44
-
beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30┬░C Escherichia coli
332
-
beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30┬░C Escherichia coli