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Literature summary for 2.7.7.18 extracted from

  • Forero-Baena, N.; Sanchez-Lancheros, D.; Buitrago, J.; Bustos, V.; Ramirez-Hernandez, M.
    Identification of a nicotinamide/nicotinate mononucleotide adenylyltransferase in Giardia lamblia (GlNMNAT) (2015), Biochim. Open, 1, 61-69.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes glnmnat-a and glnmnat-b, encoded on chromosome 3 and 5, respectively, DNA and amino acid sequence determination and analysis, phylogenetic analysis of GlNMNAT isoenzymes, sequence comparisons to human isozymes, recombinant expression of His-tagged glnmnat-a gene product in Escherichia coli strain BL21 Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics are observed for NMN and ATP, but saturation is not accomplished with NAMN, implying low affinity yet detectable activity with this substrate. Double-reciprocal plots show no cooperativity for this enzyme Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25400
-
-
Giardia intestinalis
28100
-
-
Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinate beta-D-ribonucleotide Giardia intestinalis
-
diphosphate + deamido-NAD+
-
?
additional information Giardia intestinalis bifunctional enzyme, that also shows nicotinamide-nucleotide adenylyltransferase activity, EC 2.7.7.1 ?
-
?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis A8BIC5 WB clone C6, genes glnmnat-a and glnmnat-b
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinate beta-D-ribonucleotide
-
Giardia intestinalis diphosphate + deamido-NAD+
-
?
additional information bifunctional enzyme, that also shows nicotinamide-nucleotide adenylyltransferase activity, EC 2.7.7.1 Giardia intestinalis ?
-
?

Subunits

Subunits Comment Organism
? x * 25400, about, isozyme GlNMNAT-A, sequence calculation, x * 28100, about, isozyme GlNMNAT-B, sequence calculation Giardia intestinalis
More GlNMNAT tertiary structure model with a Rossmann-type fold, overview Giardia intestinalis

Synonyms

Synonyms Comment Organism
GlNMNAT
-
Giardia intestinalis
nicotinamide/nicotinate mononucleotide adenylyltransferase
-
Giardia intestinalis
NMNAT
-
Giardia intestinalis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
26
-
assay at Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Giardia intestinalis

General Information

General Information Comment Organism
metabolism the enzyme is essentially involved in the NAD biosynthesis via two different pathways, overview Giardia intestinalis