Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.18 extracted from

  • Wagner, R.; Wagner, K.G.
    The pyridine-nucleotide cycle in tobacco. Enzyme activities for the de-novo synthesis of NAD (1985), Planta, 165, 532-537.
    View publication on PubMed

General Stability

General Stability Organism
phosphate buffer, 0.08 M, pH 7.5, stabilizes Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
nicotinate ribonucleotide 30┬░C, pH 7.5 Nicotiana tabacum
4
-
ATP 30┬░C, pH 7.5 Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinate ribonucleotide Nicotiana tabacum
-
diphosphate + deamido-NAD+
-
r

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
tobacco, cv. Samsun or cv. Xanthi
-

Source Tissue

Source Tissue Comment Organism Textmining
callus cultured from seedlings' roots Nicotiana tabacum
-
root cv. Samsun Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.00133
-
Xanthi, callus culture Nicotiana tabacum
0.00269
-
Samsun, callus culture Nicotiana tabacum
0.00358
-
Samsun, root Nicotiana tabacum

Storage Stability

Storage Stability Organism
frozen 24 h Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinate ribonucleotide
-
Nicotiana tabacum diphosphate + deamido-NAD+
-
r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Nicotiana tabacum diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Nicotiana tabacum