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Literature summary for extracted from

  • Wagner, R.; Wagner, K.G.
    The pyridine-nucleotide cycle in tobacco. Enzyme activities for the de-novo synthesis of NAD (1985), Planta, 165, 532-537.
    View publication on PubMed

General Stability

General Stability Organism
phosphate buffer, 0.08 M, pH 7.5, stabilizes Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
nicotinate ribonucleotide 30┬░C, pH 7.5 Nicotiana tabacum
ATP 30┬░C, pH 7.5 Nicotiana tabacum


Metals/Ions Comment Organism Structure
Mg2+ requirement Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinate ribonucleotide Nicotiana tabacum
diphosphate + deamido-NAD+


Organism UniProt Comment Textmining
Nicotiana tabacum
tobacco, cv. Samsun or cv. Xanthi

Source Tissue

Source Tissue Comment Organism Textmining
callus cultured from seedlings' roots Nicotiana tabacum
root cv. Samsun Nicotiana tabacum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
Xanthi, callus culture Nicotiana tabacum
Samsun, callus culture Nicotiana tabacum
Samsun, root Nicotiana tabacum

Storage Stability

Storage Stability Organism
frozen 24 h Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinate ribonucleotide
Nicotiana tabacum diphosphate + deamido-NAD+
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Nicotiana tabacum diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
assay at Nicotiana tabacum