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Literature summary for 2.7.7.15 extracted from

  • Marton, L.; Hajdu, F.; Nagy, G.N.; Kucsma, N.; Szakacs, G.; Vertessy, B.G.
    Heterologous expression of CTP phosphocholine cytidylyltransferase from Plasmodium falciparum rescues Chinese Hamster Ovary cells deficient in the Kennedy phosphatidylcholine biosynthesis pathway (2018), Sci. Rep., 8, 8932 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.15

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum rescues Chinese Hamster Ovary cells deficient in the Kennedy phosphatidylcholine biosynthesis pathway Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
H45N/H630N inactive enzyme Plasmodium falciparum
H630N mutation diminishes the catalytic rate constant of the enzyme Plasmodium falciparum
R96H/R681H thermosensitive mutant Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 CTP pH and temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
0.28
-
 CTP pH and temperature not specified in the publication, H630N mutant Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + phosphocholine Plasmodium falciparum
-
 diphosphate + CDP-choline
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IEE9
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme H630N Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phosphocholine
-
 Plasmodium falciparum diphosphate + CDP-choline
-
 ?

Synonyms

Synonyms Comment Organism
CCT
-
 Plasmodium falciparum
CTP:phosphocholine cytidylyltransferase
-
 Plasmodium falciparum
PF3D7_1316600
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
 CTP H630N mutant, pH and temperature not specified in the publication Plasmodium falciparum
1.45
-
 CTP wild-type enzyme, pH and temperature not specified in the publication Plasmodium falciparum

General Information

General Information Comment Organism
metabolism rate-limiting enzyme of the lipid biosynthesis pathway Plasmodium falciparum