Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphatidylglycerol | - |
Drosophila melanogaster | |
additional information | phosphatidylethanolamine and phosphatidic acid do not have a significant effect on CCT1 activity | Drosophila melanogaster | |
oleate | - |
Drosophila melanogaster | |
phosphatidylcholine | 6fold enhancement of activity at 0.025 mM | Drosophila melanogaster | |
phosphatidylglycerol | - |
Drosophila melanogaster | |
phosphatidylinositol | - |
Drosophila melanogaster | |
phosphatidylserine | - |
Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
expressed in Sf9 cells and Schneider 2 cells | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
choline phosphate | isozyme CCT2 | Drosophila melanogaster | |
0.81 | - |
CTP | isozyme CCT2 | Drosophila melanogaster | |
1.21 | - |
CTP | isozyme CCT1 | Drosophila melanogaster | |
2.29 | - |
choline phosphate | isozyme CCT1 | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | isozyme CCT2 | Drosophila melanogaster | 5737 | - |
nucleus | isozyme CCT1 | Drosophila melanogaster | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ yields approximately 9% of the activity seen with Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ | Drosophila melanogaster | |
Co2+ | Co2+ results in significant activity at a concentration of 25 mM, with 41% of the activity seen with 25 mM Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ | Drosophila melanogaster | |
Mg2+ | the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ | Drosophila melanogaster | |
Mn2+ | Mn2+ results in significant activity at a concentration of 25 mM, with 51% of the activity seen with 25 mM Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ | Drosophila melanogaster | |
Ni2+ | Ni2+ yields approximately 9% of the activity seen with Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ | Drosophila melanogaster | |
Zn2+ | Zn2+ results in catalytic activity of only 1% when compared to the activity in the presence of Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q7K4C7 | - |
- |
Drosophila melanogaster | Q9W0D9 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity column chromatography | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + choline phosphate | - |
Drosophila melanogaster | diphosphate + CDP-choline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCT1 | isozyme | Drosophila melanogaster |
CCT2 | isozyme | Drosophila melanogaster |
CTP:phosphocholine cytidylyltransferase | - |
Drosophila melanogaster |