Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.15 extracted from

  • Choy, P.C.; Lim, P.H.; Vance, D.E.
    Purification and characterization of CTP: cholinephosphate cytidylytransferase from rat liver cytosol (1977), J. Biol. Chem., 252, 7673-7677.
    View publication on PubMed
Search for more literature for 2.7.7.15

General Stability

General Stability Organism
freezing and thawing once causes more than 90% loss of activity Rattus norvegicus
purified enzyme is unstable in presence of DTT. CTP-Mg2+ or bovine serum albumin do not significantly stabilize the enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 diphosphate L-form, pH 6.4, 37°C Rattus norvegicus
0.007
-
 diphosphate H-form, pH 6.4, 37°C Rattus norvegicus
0.167
-
 choline phosphate L-form, pH 6.4, 37°C Rattus norvegicus
0.18
-
 choline phosphate H-form, pH 6.4, 37°C Rattus norvegicus
0.208
-
 CTP L-form, pH 6.4, 37°C Rattus norvegicus
0.21
-
 CDPcholine L-form, pH 6.4, 37°C Rattus norvegicus
0.296
-
 CTP H-form, pH 6.4, 37°C Rattus norvegicus
0.64
-
 CDPcholine H-form, pH 6.4, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 L-form of enzyme, gel filtration, aggregates in the cytosol to form high molecular weight species (H-form) with a median value of 1200000 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography, 2 forms: L-form and H-form, L-form: major species in fresh cytosol, H-form: consists of multiple copies of L-form Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.106
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate highly specific for phosphocholine Rattus norvegicus diphosphate + CDPcholine
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rattus norvegicus