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Literature summary for 2.7.7.13 extracted from

  • Zhao, S.; Liu, L.
    Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 (2016), Acta Crystallogr. Sect. F, 72, 795-798 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
ligand-free enzyme, sitting drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M lithium sulfate. Enzyme bound to GTP and D-mannose 1-phosphate, sitting drop vapor diffusion method, using 0.1 M magnesium formate, 15% (w/v) PEG 3350 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Arabidopsis thaliana
-
 diphosphate + GDP-D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O22287
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate
-
 Arabidopsis thaliana diphosphate + GDP-D-mannose
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CYT1
-
 Arabidopsis thaliana
GDP-D-mannose pyrophosphorylase
-
 Arabidopsis thaliana
GMPP
-
 Arabidopsis thaliana
VTC1 gene name, vitamin C defective 1 Arabidopsis thaliana