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Literature summary for 2.7.7.13 extracted from
- The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2007), Biochem. Biophys. Res. Commun., 353, 200-206 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Burkholderia cepacia |
| expression in of the His-tagged enzyme in Escherichia coli | Burkholderia cepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no activity is detected when 2 mM EDTA is added to the dialysis buffer | Burkholderia cepacia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
GDP-mannose | pH 7.6, temperature not specified in the publication | Burkholderia cepacia | |
| 2.9 | - |
GDP-alpha-D-mannose | pH 7.6, 10 mM Mn2+, temperature not specified in the publication | Burkholderia cepacia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | GMP activity is specifically dependent on the presence of either Mn2+ or Ca2+ | Burkholderia cepacia | |
| Ca2+ | GDP-mannose pyrophosphorylase is strongly dependent on the presence of Ca2+ or Mn2+ | Burkholderia cepacia | |
| Mn2+ | GMP activity is specifically dependent on the presence of either Mn2+ or Ca2+ | Burkholderia cepacia | |
| Mn2+ | GDP-mannose pyrophosphorylase is strongly dependent on the presence of Ca2+ or Mn2+ | Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Burkholderia cepacia |
| 55300 | - |
calculated from sequence | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | Q06XM8 | - |
- |
| Burkholderia cepacia IST408 | Q06XM8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + GDP-alpha-D-mannose | - |
Burkholderia cepacia | GTP + alpha-D-mannose 1-phosphate | - |
? | |
| diphosphate + GDP-alpha-D-mannose | - |
Burkholderia cepacia IST408 | GTP + alpha-D-mannose 1-phosphate | - |
? | |
| diphosphate + GDP-mannose | - |
Burkholderia cepacia | GTP + alpha-D-mannose 1-phosphate | - |
? | |
| diphosphate + GDP-mannose | - |
Burkholderia cepacia IST408 | GTP + alpha-D-mannose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Burkholderia cepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BceA | - |
Burkholderia cepacia |
| GDP-mannose pyrophosphorylase | - |
Burkholderia cepacia |
| More | bifunctional protein (type II PMI family) with phosphomannose isomerase (PMI, EC 5.3.1.8) and GDP-mannose pyrophosphorylase (GMP, EC 2.7.7.13) activities | Burkholderia cepacia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 195.9 | - |
GDP-alpha-D-mannose | pH 7.6, 10 mM Mn2+, temperature not specified in the publication | Burkholderia cepacia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Burkholderia cepacia |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Burkholderia cepacia | calculated from sequence | - |
6.03 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation | Burkholderia cepacia |
| metabolism | the bceA gene is part of the exopolysaccharide biosynthetic cluster | Burkholderia cepacia |
| physiological function | the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed | Burkholderia cepacia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 195.9 | - |
GDP-mannose | pH 7.6, temperature not specified in the publication | Burkholderia cepacia | |
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