Cloned (Comment) | Organism |
---|---|
expression of GST-GDPmannose pyrophosphorylase fusion protein in Escherichia coli | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000039 | - |
GDPmannose | pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate diphosphate | Sus scrofa | |
0.2 | - |
mannose 1-phosphate | pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate GTP | Sus scrofa | |
0.36 | - |
GTP | pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate mannose 1-phosphate | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | recombinant beta subunit of GDPmannose pyrophosphorylase, 50% of activation with Mn2+ | Sus scrofa | |
Mn2+ | best metal activator of recombinant beta subunit of GDPmannose pyrophosphorylase, maximal activation at 0.5 mM | Sus scrofa | |
additional information | recombinant beta subunit of GDPmannose pyrophosphorylase is not activated by Co2+, Zn2+, Cu2+ and Fe2+ | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
43000 Da alpha subunit or a combinantion of alpha and beta subunit constitutes the GDPglucose pyrophosphorylase activity, the 37000 Da beta subunit constituts the GDPmannose pyrophosphorylase activity | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Sus scrofa | - |
GDPmannose + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-mannose 1-phosphate | recombinant beta subunit of GDPmannose pyrophosphorylase, 18% of activity with GTP | Sus scrofa | ADPmannose + diphosphate | - |
? | |
CTP + alpha-D-mannose 1-phosphate | recombinant beta subunit of GDPmannose pyrophosphorylase, 7% of activity with GTP | Sus scrofa | diphosphate + CDP-alpha-D-mannose | - |
? | |
GTP + alpha-D-mannose 1-phosphate | - |
Sus scrofa | GDPmannose + diphosphate | - |
? | |
GTP + alpha-D-mannose 1-phosphate | recombinant 37000 Da beta subunit of GDP-mannose pyrophosphorylase has only little GDPglucose synthetic activity but high GDP mannose synthetic activity | Sus scrofa | GDPmannose + diphosphate | - |
? | |
ITP + alpha-D-mannose 1-phosphate | recombinant beta subunit of GDPmannose pyrophosphorylase, 20% of activity with GTP | Sus scrofa | IDPmannose + diphosphate | - |
? | |
UTP + alpha-D-mannose 1-phosphate | recombinant beta subunit of GDPmannose pyrophosphorylase, 12% of activity with GTP | Sus scrofa | UDPmannose + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Sus scrofa |
More | 43000 Da alpha subunit or a combinantion of alpha and beta subunit constitutes the GDPglucose pyrophosphorylase activity, the 37000 Da beta subunit constituts the GDPmannose pyrophosphorylase activity | Sus scrofa |