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Literature summary for 2.7.7.13 extracted from

  • Ning, B.; Elbein, A.D.
    Cloning, expression and characterization of the pig liver GDP-mannose pyrophosphorylase: evidence that GDP-mannose and GDP-Glc pyrophosphorylases are different proteins (2000), Eur. J. Biochem., 267, 6866-6874.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-GDPmannose pyrophosphorylase fusion protein in Escherichia coli Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000039
-
GDPmannose pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate diphosphate Sus scrofa
0.2
-
mannose 1-phosphate pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate GTP Sus scrofa
0.36
-
GTP pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate mannose 1-phosphate Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ recombinant beta subunit of GDPmannose pyrophosphorylase, 50% of activation with Mn2+ Sus scrofa
Mn2+ best metal activator of recombinant beta subunit of GDPmannose pyrophosphorylase, maximal activation at 0.5 mM Sus scrofa
additional information recombinant beta subunit of GDPmannose pyrophosphorylase is not activated by Co2+, Zn2+, Cu2+ and Fe2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
43000 Da alpha subunit or a combinantion of alpha and beta subunit constitutes the GDPglucose pyrophosphorylase activity, the 37000 Da beta subunit constituts the GDPmannose pyrophosphorylase activity Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
GDPmannose + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 18% of activity with GTP Sus scrofa ADPmannose + diphosphate
-
?
CTP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 7% of activity with GTP Sus scrofa diphosphate + CDP-alpha-D-mannose
-
?
GTP + alpha-D-mannose 1-phosphate
-
Sus scrofa GDPmannose + diphosphate
-
?
GTP + alpha-D-mannose 1-phosphate recombinant 37000 Da beta subunit of GDP-mannose pyrophosphorylase has only little GDPglucose synthetic activity but high GDP mannose synthetic activity Sus scrofa GDPmannose + diphosphate
-
?
ITP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 20% of activity with GTP Sus scrofa IDPmannose + diphosphate
-
?
UTP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 12% of activity with GTP Sus scrofa UDPmannose + diphosphate
-
?

Subunits

Subunits Comment Organism
?
-
Sus scrofa
More 43000 Da alpha subunit or a combinantion of alpha and beta subunit constitutes the GDPglucose pyrophosphorylase activity, the 37000 Da beta subunit constituts the GDPmannose pyrophosphorylase activity Sus scrofa