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Literature summary for 2.7.7.13 extracted from

  • Szumilo, T.; Drake, R.R.; York, J.L.; Elbein, A.D.
    GDP-mannose pyrophosphorylase. Purification to homogeneity, properties, and utilization to prepare photoaffinity analogs (1993), J. Biol. Chem., 268, 17943-17950.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Sus scrofa
GDP 0.4 mM, 50% inhibition Sus scrofa
GDP-glucose inhibition of GDP-mannose phosphorolysis Sus scrofa
GDP-mannose noncompetitive inhibition of GDP-glucose phosphorolysis Sus scrofa
glucose 1-phosphate inhibition of GDP-mannose phosphorolysis Sus scrofa
GMP 0.4 mM, 50% inhibition Sus scrofa
Hg2+
-
Sus scrofa
mannose 1-phosphate noncompetitive inhibition of GDP-glucose phosphorolysis Sus scrofa
Zn2+
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
GDPmannose pH 7.5, 37°C Sus scrofa
0.1
-
GTP pH 7.5, 37°C, cosubstrate mannose 1-phosphate Sus scrofa
0.13
-
mannose 1-phosphate pH 7.5, 37°C, cosubstrate GTP Sus scrofa
0.33
-
diphosphate pH 7.5, 37°C, cosubstrate GDPglucose Sus scrofa
0.5
-
diphosphate pH 7.5, 37°C, cosubstrate GDPmannose Sus scrofa
1
-
GTP pH 7.5, 37°C, cosubstrate glucose 1-phosphate Sus scrofa
2.85
-
glucose 1-phosphate pH 7.5, 37°C, cosubstrate GTP Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight activation at about 10 mM Sus scrofa
Mg2+ maximal activation at 5 mM Sus scrofa
Mn2+ 70% of activity with Mg2+, inhibition at 10 mM and higher Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000 + x * 43000 SDS-PAGE Sus scrofa
43000
-
x * 37000 + x * 43000 SDS-PAGE Sus scrofa
450000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
GDPmannose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acid treatment, protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxylapatite, Blue Sepharose Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.25
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
frozen, 20 mM Tris-HCl, pH 7.5, 1 mM 2-mercaptoethanol, 1 mM EDTA, several months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-Azido-GTP + glucose 1-phosphate
-
Sus scrofa 8-Azido-GDPglucose + diphosphate
-
?
8-Azido-GTP + mannose 1-phosphate
-
Sus scrofa 8-Azido-GDP mannose + diphosphate
-
?
GDP-glucose + diphosphate most effective substrate in direction of nucleoside triphosphate formation, in the reverse direction GTP is a better glucose acceptor than ITP Sus scrofa D-glucose 1-phosphate + GTP
-
r
GDPmannose + diphosphate 61% of the activity with GDPglucose Sus scrofa ?
-
r
GTP + alpha-D-mannose 1-phosphate
-
Sus scrofa GDPmannose + diphosphate
-
r
IDPmannose + diphosphate 72% of activity with GDPglucose Sus scrofa ITP + mannose 1-phosphate
-
r
ITP + alpha-D-mannose 1-phosphate ITP is more effective than GTP with mannose 1-phosphate Sus scrofa IDPmannose + diphosphate
-
r
ITP + glucose 1-phosphate
-
Sus scrofa IDP-glucose + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 37000 + x * 43000 SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 GTP synthesis Sus scrofa
6 8.5 GDPmannose synthesis Sus scrofa
8.2
-
assay at Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.7 most stable at Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.024
-
GDP-mannose pH 7.5, 37°C Sus scrofa
0.06
-
mannose 1-phosphate pH 7.5, 37°C Sus scrofa
2.5
-
glucose 1-phosphate pH 7.5, 37°C Sus scrofa
11
-
GDP-glucose pH 7.5, 37°C Sus scrofa