Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Sus scrofa | |
GDP | 0.4 mM, 50% inhibition | Sus scrofa | |
GDP-glucose | inhibition of GDP-mannose phosphorolysis | Sus scrofa | |
GDP-mannose | noncompetitive inhibition of GDP-glucose phosphorolysis | Sus scrofa | |
glucose 1-phosphate | inhibition of GDP-mannose phosphorolysis | Sus scrofa | |
GMP | 0.4 mM, 50% inhibition | Sus scrofa | |
Hg2+ | - |
Sus scrofa | |
mannose 1-phosphate | noncompetitive inhibition of GDP-glucose phosphorolysis | Sus scrofa | |
Zn2+ | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
GDPmannose | pH 7.5, 37°C | Sus scrofa | |
0.1 | - |
GTP | pH 7.5, 37°C, cosubstrate mannose 1-phosphate | Sus scrofa | |
0.13 | - |
mannose 1-phosphate | pH 7.5, 37°C, cosubstrate GTP | Sus scrofa | |
0.33 | - |
diphosphate | pH 7.5, 37°C, cosubstrate GDPglucose | Sus scrofa | |
0.5 | - |
diphosphate | pH 7.5, 37°C, cosubstrate GDPmannose | Sus scrofa | |
1 | - |
GTP | pH 7.5, 37°C, cosubstrate glucose 1-phosphate | Sus scrofa | |
2.85 | - |
glucose 1-phosphate | pH 7.5, 37°C, cosubstrate GTP | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight activation at about 10 mM | Sus scrofa | |
Mg2+ | maximal activation at 5 mM | Sus scrofa | |
Mn2+ | 70% of activity with Mg2+, inhibition at 10 mM and higher | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000 + x * 43000 SDS-PAGE | Sus scrofa |
43000 | - |
x * 37000 + x * 43000 SDS-PAGE | Sus scrofa |
450000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Sus scrofa | - |
GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acid treatment, protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxylapatite, Blue Sepharose | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.25 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
frozen, 20 mM Tris-HCl, pH 7.5, 1 mM 2-mercaptoethanol, 1 mM EDTA, several months, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-Azido-GTP + glucose 1-phosphate | - |
Sus scrofa | 8-Azido-GDPglucose + diphosphate | - |
? | |
8-Azido-GTP + mannose 1-phosphate | - |
Sus scrofa | 8-Azido-GDP mannose + diphosphate | - |
? | |
GDP-glucose + diphosphate | most effective substrate in direction of nucleoside triphosphate formation, in the reverse direction GTP is a better glucose acceptor than ITP | Sus scrofa | D-glucose 1-phosphate + GTP | - |
r | |
GDPmannose + diphosphate | 61% of the activity with GDPglucose | Sus scrofa | ? | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Sus scrofa | GDPmannose + diphosphate | - |
r | |
IDPmannose + diphosphate | 72% of activity with GDPglucose | Sus scrofa | ITP + mannose 1-phosphate | - |
r | |
ITP + alpha-D-mannose 1-phosphate | ITP is more effective than GTP with mannose 1-phosphate | Sus scrofa | IDPmannose + diphosphate | - |
r | |
ITP + glucose 1-phosphate | - |
Sus scrofa | IDP-glucose + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000 + x * 43000 SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | GTP synthesis | Sus scrofa |
6 | 8.5 | GDPmannose synthesis | Sus scrofa |
8.2 | - |
assay at | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.7 | most stable at | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
GDP-mannose | pH 7.5, 37°C | Sus scrofa | |
0.06 | - |
mannose 1-phosphate | pH 7.5, 37°C | Sus scrofa | |
2.5 | - |
glucose 1-phosphate | pH 7.5, 37°C | Sus scrofa | |
11 | - |
GDP-glucose | pH 7.5, 37°C | Sus scrofa |